某院孕产妇感染性病原体血清标志物的检测结果分析

Clinical value analysis of maternal detection of serum markers of infectious pathogens

目的分析本院孕产妇的感染性病原体血清标志物的检测结果,为优生优育提供保障。方法选择2015年6月-2017年6月在菏泽市妇幼保健院就诊的6 000例孕产妇作为研究对象,采用酶联免疫吸附试验(ELISA法)检测乙型肝炎(乙肝)表面抗原(HBsAg)、丙型肝炎(丙肝)抗体和人类免疫缺陷病毒(HIV)抗体,对HBsAg阳性患者的血清进一步检测乙肝5项〔HBsAg、乙肝表面抗体(HBsAb)、e抗原(HBeAg)、e抗体(HBeAb)、核心抗体(HBcAb)〕;采用甲苯胺红不加热血清试验(TRUST)检测梅毒抗体;观察所有检测结果并进行统计学分析。结果共纳入6 000例孕产妇。HBsAg阳性率为5.0%(300/6 000);丙肝抗体阳性率为0.1%(6/6 000);HIV抗体初筛阳性率为0.07%(4/6 000),均经获得性免疫缺陷综合征(AIDS)实验室确认;梅毒阳性率为0.03%(2/6 000),均经滴度试验确认。"小三阳"阳性率为50.33%,"大三阳"阳性率为37.67%;其中HBcAb阳性率最高(90.24%),其次为HBeAb(50.65%)和HBeAg(46.49%),HBsAb阳性率最低(0.72%)。结论本院存在一定程度的孕产妇感染,尤其以HBsAg阳性患者居多,做好检测和预防是控制感染的重要途径,临床应引起重视。

Objective To analyze the detection results of serum markers of infectious pathogens in pregnant and lying-in women in our hospital so as to provide guarantee for eugenic and healthy birth. Methods Six thousand pregnant and lying-in women who visited Heze Maternal and Child Health Hospital from June 2015 to June 2017 were selected as the study subjects. Hepatitis B surface antigen(HBsAg), hepatitis C antibody(HCV-Ab)and human immunodeficiency virus(HIV) antibody were detected by enzyme linked immunosorbent assay(ELISA),and then the five markers of hepatitis B in the serum of patients with HBsAg positive were further detected [HBsAg,hepatitis B surface antibody(HBsAb), hepatitis Be antigen(HBeAg), hepatitis Be antibody(HBeAb), core antibody(HBc Ab)];syphilis antibody was detected by toluidine red unheated serum test(TRUST);all the results were observed and analyzed statistically. Results A total of 6 000 pregnant and lying-in women were enrolled. The positive rate of HBsAg was 5.0%(300/6 000);the positive rate of hepatitis C antibody was 0.1%(6/6 000);the initial screen positive rate of HIV antibody was 0.07%(4/6 000), and all were confirmed by acquired immunodeficiency syndrome(AIDS) Laboratory;the positive rate of syphilis was 0.03(2/6 000), all being definitely recognized after titration tests.The positive rate of "Xiaosanyang" was 50.33%, and that of "Dasanyang" was 37.67%;among them HBcAb positive rate was the highest(90.24%), followed by HBeAb(50.65%) and HBeAg(46.49%) and HBsAb positive rate was the lowest(0.72%). Conclusions There is a certain degree of infectious pathogen infection in pregnant and lying-in women, especially the number of patients with positive HBsAg being in a greater amount. Detection and prevention ought to be well performed, which are the important ways to control the infection and should be paid attention to clinically.