HCV抗体血清学检测联合HCV-RNA检测的应用价值:附2例病例分析

Application value of anti-HCV serological detection combined with HCV-RNA detection-analysis of two cases

丙型肝炎(丙肝)为常见传染病,丙肝病毒(HCV)抗体血清学检测是HCV感染筛查的主要方式,常用的方法为化学发光法。但感染HCV后,抗体的血清学转换可延迟至数月后。HCV-RNA检测可直接检测病毒复制量,感染HCV后1~2周即可在血清中检测到病毒,是HCV感染的实验室诊断方法之一。但HCV-RNA检测操作繁琐、成本较高,常规实验室不作为筛选使用。威海市立医院收治2例疑诊病毒性肝炎患者,经核酸检测确诊后即给予相应治疗,患者肝功能好转。说明在实验室常规开展HCV-RNA检测提高了HCV感染检出的准确性。

Hepatitis C is a common infectious disease. Serological detection of hepatitis C virus(HCV antibody is the main method for HCV infection screening, and the common method of HCV antibody detection is chemiluminescence. However, the serological conversion of antibodies can be delayed for several months after infection with HCV. The HCV-RNA detection can directly measure the viral replication amount, and the virus can be detected in serum within 1 to 2 weeks after infection with HCV, which is one of the laboratory diagnostic methods for HCV infection. But the HCV-RNA detection is cumbersome and costly, so conventional laboratories do not use it for screening. Weihai Municipal Hospital admitted and treated 2 patients with suspected viral hepatitis. After confirmation of the HCV diagnosis by the nucleic acid test, the patients were given corresponding treatment and their liver functions were improved, indicating that routine HCV-RNA detection in the laboratory can improve the accuracy of HCV infection detection.