β-榄香烯对体外培养恶性疟原虫生长抑制作用的实验研究

Study on Suppression Effect of Beta-elemene against Plasmodium Falciparum in Vitro

目的:研究从中药莪术中分离得到的新型非细胞毒性抗肿瘤药物β-榄香烯的体外抗疟活性,。方法:采用恶性疟原虫3D7株(氯喹敏感株)和Dd2株(氯喹抗性株)进行测试,恶性疟原虫经过同步化处理后添加β-榄香烯,40 h后涂片镜检,观察其细胞形态变化;采用SYBR Green I染料法检测两种虫株对β-榄香烯和氯喹的半数抑制浓度(IC50)。结果:与空白对照组相比,添加10μg/mL的β-榄香烯能抑制恶性疟原虫的生长,40μg/mL的β-榄香烯可以完全抑制恶性疟原虫的生长;3D7虫株和Dd2虫株对β-榄香烯的IC50值分别为(13.60±0.540)μg/mL和(12.59±0.54)μg/mL。结论:β-榄香烯具有显著的体外抗疟活性,值得进一步深入研究。

Objective: To study the antimalarial activity of β-elemene in vitro, a novel non-cytotoxic antitumor drug isolated from the Traditional Chinese Medicinal herb Rhizoma Zedoariae. Methods: Chloroquine(CQ)-sensitive(3 D7)and CQ-resistant(Dd2) strains of Plasmodium falciparum were tested using β-elemene and the fluorescence-based SYBR Green I assay was used to calculate the growth inhibition. Results: Compared with non-treated control,β-elemene can arrest the parasites growth at 10 μg/mL,and the parasites could be significantly inhibited at 40 μg/mL. The 50% inhibitory concentration(IC50) values of β-elemene were(13.60±0.540)μg/mL for 3 D7 and(12.59±0.54)μg/mL for Dd2,respectively. Conclusion: These results demonstrated that β-elemene showed good antimalarial effect, and the β-elemene seemed to be of special interest for further antimalarial studies.