无菌部位分离65株金黄色葡萄球菌耐药情况及耐药机制

Drug resistance and mechanism of 65 strains of Staphylococcus aureus isolated from aseptic sites

目的研究无菌部位分离的金黄色葡萄球菌(金葡菌)对红霉素、克林霉素耐药率及耐药机制。方法微量肉汤稀释法检测金葡菌3种抗生素的最小抑菌浓度以及克林霉素诱导试验,采用PCR检测4种耐药基因。结果65株金葡菌中,共检出耐甲氧西林金葡菌(MRSA)35株,甲氧西林敏感金葡菌(MSSA)30株,MRSA检出率为53.8%;对红霉素、克林霉素耐药率分别为67.7%、33.8%;MRSA的红霉素、克林霉素耐药率均高于MSSA,差异有统计学意义(P值均<0.05)。克林霉素诱导耐药率为95.5%。耐药基因ermC携带率最高,MRSA携带耐药基因数高于MSSA。结论无菌部位分离的金葡菌对红霉素和克林霉素耐药率较高,且MRSA比MSSA携带有更多的耐药基因。

Objective To understand the resistance rates and mechanism of Staphylococcus aureus strains isolated from sterile sites to erythromycin and clindamycin.Methods Micro-dilution broth method was used to determine the antimicrobial minimal inhibitory concentrations to 3antibiotic drugs and conduct clindamycin induction test;the 4drug resistant genes were detected by PCR.Results Among 65strains of Staphylococcus aureus,a total of 35strains of methicillin-resistant Staphylococcus aureus (MRSA)and 30strains of methicillin-sensitive Staphylococcus aureus (MSSA)were detected,resulting MRSA detection rate of 53.8%.The drug resistant rates to erythromycin and clindamycin were 67.7%and 33.8%,respectively.The drug resistant rates of MRSA to both erythromycin and clindamycin were higher than those of MSSA,with significantly statistical differences(all P<0.05).The induced drug resistant rate to clindamycin was 95.5%.Drug resistance gene ermC had the highest positive rates while MRSA isolates carried more resistance genes than MSSA isolates.Conclusion The Staphylococcus aureus strains isolated from sterile sites have high drug resistant rates to erythromycin and clindamycin.MRSA isolates carry more resistance genes than MSSA isolates.