‘西伯利亚’百合钙依赖型蛋白激酶基因的克隆及表达分析

Cloning and Expression Analysis of Calcium-Dependent Protein Kinase Gene in Lilium ’Siberia’

钙依赖型蛋白激酶(calcium-dependent protein kinase, CDPK)是一类Ser/Thr型蛋白激酶,在植物体内钙信号的传递中起着重要的作用。本研究以东方百合‘西伯利亚’(Oriental Lilium’siberia’)为材料,通过RACE方法克隆了LiCDPK基因,并进行了时空表达分析。结果表明,百合LiCDPK基因全长2 231 bp,开放阅读框长1 551 bp,编码516个氨基酸,其蛋白序列与其他物种的CDPK蛋白相似性在80%以上。LiCDPK基因在不同花期和9个不同组织中的表达存在差异。不同花期的qRT-PCR表明,花蕾期LiCDPK基因的表达量最高;盛花期的不同组织器官中,花药LiCDPK基因的表达量最高。本研究为进一步揭示LiCDPK基因在百合花香释放过程中的信号作用提供了帮助。

Calcium-dependent protein kinase(CDPK) is a type of Ser/Thr protein kinases, which plays an important role in signaling transduction of calcium in plants. In this study, the LiCDPK gene was cloned by RACE technique using oriental Lilium ’Siberia’ as materials, and the temporal and spatial expression were analysed. The results showed that the whole length of LiCDPK was 2 231 bp, ORF was 1 551 bp, encoding 516 amino acids, and its protein sequence was more than 80% similar to that of other species. The expression of LiCDPK presented different levels in different flowering stages and nine different tissues. The qRT-PCR of different flowering stages revealed that the expression level of LiCDPK was the highest at the bud stage. Among the different tissues of full bloom stage, the highest expression level of LiCDPK was found in anther. This study could be helpful for further studies on the signal function of LiCDPK in the process of fragrance release of Lilium.