电针对蛛网膜下腔出血大鼠脑血流灌注影响与神经功能障碍的关联研究

A study on the effect of electroacupuncture on cerebral blood flow and neurological dysfunction in rats with subarachnoid hemorrhage

目的:研究电针对蛛网膜下腔出血大鼠不同时期针刺对SAH大鼠脑血流灌注的影响。方法:将90只SD大鼠随机分为假手术组、模型组、电针组、尼莫地平组、尼莫地平+电针组,每组各18只。采用Bederson法建立SAH模型,于造模后第1、3、5、7天分别对各亚组大鼠进行Loeffler神经行为学评分及Morris水迷宫逃避潜伏期测定,并检测相应时间点大鼠皮质脑血流。结果:电针组与模型组比较,第3、5、7天rCBF有显著差异(P<0.05),第5、7天Loeffler评分及逃避潜伏期有显著差异(P<0.05)。尼莫地平+电针组与电针组及尼莫地平组比较,第1、3、5、7天rCBF有显著差异(P<0.05),第3、5、7天Loeffler评分有显著差异(P<0.05),第5、7天逃避潜伏期有显著差异(P<0.05)。结论:针刺大鼠百会、神庭穴可明显改善SAH大鼠脑血管痉挛增加脑血流灌注,从而改善认知功能障碍及行为学功能障碍。

Objective:To study the effect of electroacupuncture on cortical blood flow in subarachnoid hemorrhage rats at different stages. Method:Totally 90 SD rats were randomly divided into sham operation group, model group, electroacupuncture group, Nimodipine group, Nimodipine+electroacupuncture group, with 18 rats in each group. Subarachnoid hemorrhage( SAH) model was established by Bederson's method. Neurobehavioral scores of rats in each subgroup were scored on the 1st, 3rd, 5th, 7th day after model establishment, and the cortical cerebral blood flow was measured at the corresponding time point. Result:The rCBF of electroacupuncture group was significantly different from that of the model group at 3, 5 and 7 days (P<0.05), and the Loeffler score and escape latency at 5 and 7 days were significantly different (P<0.05). The rCBF was significantly different on the 1st, 3rd, 5th and 7th days (P<0.05), and the Loeffler score was significantly different on the 3rd, 5th and 7th days (P<0.05), and the escape latency was significantly different on the 5th and 7th day (P<0.05). Conclusion:Electroacupuncture at Baihui and Shenting can significantly improve SAH rat cerebral vasospasm and increase cortical blood flow, thereby improving cognitive dysfunction and behavioral dysfunction.