硼对厌氧氨氧化反应器启动过程及菌群结构的影响

Effect of Boron on Start-up Process and Microbial Community in Anaerobic Ammonium Oxidation Reactor

第二类自诱导物型群体感应是细菌本身能够分泌一种信号分子(A1-2)并感应其浓度,进而调节特定基因表达及控制菌群行为的现象,比如生物膜的形成。硼是AI-2的重要组分,因而通过在厌氧氨氧化反应器启动过程中投加一定量硼酸(0.24 mmol/L),探讨硼对厌氧氨氧化反应器启动及菌群结构的影响以城市污水处理厂的活性污泥作为反应器接种污泥,并控制pH值和温度分别在7.5~7.7、(34±1)℃,经过154d运行,总氮去除负荷达到1.77 kgN/(m^3·d),总氮去除率在88.3%左右,高于其他文献报道的相同反应器的总氮去除负荷。Illumina MiSeq高通量测序结果显示,经过154(I的运行,反应器中的优势菌群为浮霉菌门(26.60%)、变形菌门(23.41%)、绿弯菌门(29.81%),其中优势厌氧氨氧化菌为Candidate Jettenia ,占21.07%。

Autoinducer-2 ( AI-2 ) mediated quorum sensing is a system that the bacteria can secrete a signal molecule and detect its concentration to regulate specific gene expression and coordinate bacterial behaviors such as biofilm formation. Boron (B) is an important component of AI-2. A certain amount of H3BO3 (0.24 mmol/L) was added to anaerobic ammonium oxidation ( Anammox ) reactor during the start-up process to investigate the effect of boron on the start-up and microbial community stixicture. The reactor was inoculated with activated sludge from municipal wastewater treatment plant, and the pH value and temperature were controlled at 7.5 - 7.7 and (34±1)℃, respectively. After 154 days' operation, the total nitrogen removal load of the reactor reached 1.77 kgN/(m^3·d), and the total nitrogen removal efficiency reached 88.3%. Illumina MiSeq high-throughput sequencing results showed that after 154 days of operation, dominant bacteria in Anammox reactor were Planetomycetes (26.60%), Proteobacteria (23.41 %) and Chloroflexi (29.81 %). The dominant Anammox bacteria was Candidatus Jettenia accounting for 21.07 %.