透明时间对草鱼脂肪组织和肌肉组织石蜡切片制作效果的影响

Effects of Different Transparent Time on the Paraffin Section’s Quality of Abdominal Adipose Tissue and Muscle Tissue in Grass Carp

【目的】探索不同透明时间对草鱼(Ctenopharyngodon idella)脂肪组织和肌肉组织石蜡切片制作效果的影响,并筛选出最佳透明时间。【方法】应用石蜡切片技术对草鱼腹腔脂肪组织和背部肌肉组织进行切片,经苏木精-伊红染色后通过显微摄影来观察每类组织在不同透明时间的切片完整率和细胞完整性。【结果】草鱼腹腔脂肪组织在连续两次透明时间分别为3,1min的条件下,切片完整率为88.33%,脂肪细胞形态完整且细胞膜无破碎;背部肌肉组织在连续两次透明时间分别为10,8min的条件下,切片完整率为93.33%,肌细胞形态完整无破损,且肌间脂肪细胞界限明显。【结论】石蜡切片的制作效果取决于组织特性,草鱼脂肪组织和肌肉组织石蜡切片连续两次的最佳透明时间分别为3,1min和10,8min。

[Purposes]This study aimed to explore the effects of different transparent time on the paraffin section’s quality of abdominal adipose tissues and back muscle tissue in grass carp(Ctenopharyngodon idella),and to screen the best transparent time to make paraffin section of the two kinds of tissues.[Methods]The abdominal adipose tissue and back muscle tissue of grass carp were sliced through the technology of paraffin section,and stained through HE staining.After that,the integrity rate of slices was calculated and the cell morphology was observed.[Findings]The best successive transparent time was 3,1 min for adipose tissue of grass carp.Under such transparent conditions,the integrity rate of slices was 88.33%,and the intact membrane and adipocytes were observed by using microscopy.After two consecutive transparent time of 10,8 min,the integrity rate of slices reached 93.33%,and muscle cells were intact without damaged,and the boundaries between intermuscular adipocytes was well-defined.[Conclusions]The making procedure of paraffin sections is determined by tissue characteristics.For the adipose tissue,the best consecutive transparent time is 3,1 min,and for the muscle tissue,the best consecutive transparent time is 10,8 min.