高效液相色谱法同时测定复方赖氨酸颗粒中4种成分的含量

HPLC simultaneous determination of 4 effective components in compound lysine granules

目的建立HPLC法同时测定复方赖氨酸颗粒中4种成分(维生素B1、维生素B6、葡萄糖酸钙和盐酸赖氨酸)的含量。方法采用氨基柱,以乙腈-0.05 mol·L^-1磷酸二氢钾溶液(68∶32)为流动相,流速为1.0 mL·min^-1,柱温为30℃,检测波长:维生素B1和维生素B6为280 nm,葡萄糖酸钙、盐酸赖氨酸为205 nm,进样量10μL。结果维生素B1、维生素B6、葡萄糖酸钙、盐酸赖氨酸分别在0.085 61~0.1998 mg·mL^-1、0.054 84~0.1280 mg·m L^-1、1.183~2.761 mg·mL^-1、2.049~4.781mg·mL^-1与峰面积线性关系良好,平均加样回收率分别为100.4%、99.7%、100.7%、100.7%,RSD分别为0.99%、0.83%、0.67%、0.80%(n=6)。结论该法准确、简单、方便、可行,可用于同时测定复方赖氨酸颗粒中4个有效成分的含量。

Objective To determine 4 effective components in compound lysine granules by HPLC.Methods An NH2 column was used with the column temperatureb at 30℃.The mobile phase consisted of acetonitrile-0.05 mol·L^-1 KH2 PO4(68∶32)at the flow rate of 1.0 mL·min^-1.The detection wavelength was 280 nm(vitamin B1 and vitamin B6)and 205 nm(lysine hydrochloride and calcium gluconate)and the injection volume was 10μL.Results The linear range of vitamin B1,vitamin B6,calcium gluconate,and lysine hydrochloride was 0.085 61-0.1998 mg·mL^-1,0.054 84-0.1280 mg·mL^-1,1.183-2.761 mg·mL^-1,and 2.049-4.781 mg·mL^-1,respectively.The average recovery(n=6)was 100.4%,99.7%,100.7%,and 100.7%with the RSD at 0.99%,0.83%,0.67%,and 0.80%.Conclusion The method is accurate,simple,convenient and feasible,which can be used for the determination of 4 effective components in compound lysine granules.