心康冲剂改善慢性心衰模型大鼠心肌凋亡及调控Caspase-3/9的表达

Xinkang Granule Improves Myocardial Apoptosis and the Expression of Caspase-3/9 in Rats with Chronic Heart Failure

目的探讨心康冲剂对心衰大鼠Caspase-3、Caspase-9表达的调控作用。方法58只SD大鼠分为正常组10只,模型组、心康组及对照组各16只,造模完后,心康组给予心康冲剂溶液0.5 g/kg灌服,对照组予芪苈强心胶囊溶液0.06 g/kg灌服。采用心脏彩超检测心功能;心脏切片行TUNNEL染色法观察心肌细胞凋亡;心肌组织采用Real-time PCR法及免疫组化法检测Caspase-3、Caspase-9基因及蛋白质表达。结果与正常组比,模型组大鼠Caspase-3及Caspase-9的mRNA与蛋白表达显著增加(P<0.01),TUNNEL染色观察细胞凋亡明显增多;与模型组比较,心康组大鼠心肌凋亡减轻,Caspase-9 mRNA、Caspase-3 mRNA及蛋白明显下降(P<0.01);与对照组相比,心康组心肌细胞凋亡相对降低,Caspase-3蛋白表达下降(P<0.05)。结论心康冲剂可调控下调Caspase-3、Caspase-9表达抗心衰。

Objective To investigate the regulatory effects of Xinkang Granule (XKG) on the rats with chronic heart failure (CHF) for the expression of Caspase-3 and Caspase-9. Methods A total of 58 SD rats were randomly divided into a normal group (n=10), a model group, a XKG group, and a control group, with 16 rats in each group. After the success of the modeling, the XKG group was given XKG solution at 0.5 g/kg, and the control group was given Qili Qiangxin Capsule (QLQXC) solution at 0.06 g/kg. Cardiac function was measured by echocardiography;Heart slices were stained by TUNNEL to observe myocardial apoptosis. The expression of gene and protein of Caspase-3, Caspase-9 were detected by Real-time PCR and immunohistochemistry. Results Compared with the normal group, the gene and protein of Caspase-3, Caspase-9 in the model group significantly increased (P<0.01). TUNNEL observed significant increase of apoptosis. Compared with the model group, the rats in XKG group showed alleviated myocardial apoptosis, and the Caspase-3, Caspase-9 mRNA and protein expression were significantly reduced (P<0.01). Compared with the control group, the myocardial apoptosis in the XKG group was relatively decreased, and Caspase-3 decreased (P<0.05). Conclusion XKG has the function of anti-heart failure by regulating and controlling the expression of Caspase-3 and Caspase-9.