DES-Fe3O4 composite for rapid extraction of residual plant growth regulators in edible vegetable oil

A rapid DES-Fe3O4 microextraction coupled with liquid chromatography method was ingeniously developed for simultaneous control of five typical plant growth regulators in a planting cycle including indole-3-acetic acid, abscisic acid, thidiazuron, 1-naphthylacetic acid and forchlorfenuron. The experiments were carried out with one type of carboxylic acid-assisted deep eutectic solvent (nChCl:nacetic acid=1:3) as extractant, coupled with ultrasonic wave extraction for 30 min at bath temperature at 50 ℃. Under the optimum conditions, good extraction performances of linearities for five plant growth regulators were achieved separately in the range of 0.10–50 mg/L of indole-3-acetic acid, 0.06–50 mg/L of abscisic acid, 0.20–50 mg/L of thidiazuron, 0.50–50 mg/L of 1-naphthylacetic acid and 0.06–50 mg/L of forchlorfenuron and recoveries were ranged from 70.4%to 102.2%. Meanwhile, Fe3O4 was preferentially combined with DES in the oil matrix, the advantage of which was pointed to rapid and efficient separation of targeted plant growth regulators from complicated oil matrix. The proposed approach achieved satisfied results and met the monitoring requirements of plant growth regulators control in edible vegetable oil samples.

A rapid DES-Fe304 microextraction coupled with liquid chromatography method was ingeniously developed for simultaneous control of five typical plant growth regulators in a planting cycle including indole-3-acetic acid,abscisic acid,thidiazuron,1-nap hthy lace tic acid and forchlorfenuron.The experiments were carried out with one type of carboxylic acid-assisted deep eutectic solvent(nchci:nacetic acid=1:3)as extractant,coupled with ultra sonic wave extraction for 30 min at bath temperature at50 ℃.Under the optimum conditions,good extraction performances of linearities for five plant growth regulators were achieved separately in the ra nge of 0.10-50 mg/L of indole-3-acetic acid,0.06-50 mg/L of abscisic acid,0.20-50 mg/L of thidiazuron,0.50-50 mg/L of 1-naphthylacetic acid and 0.06-50 mg/L of forchlorfenuron and recoveries were ranged from 70.4%to 102.2%.Meanwhile,Fe304 was preferentially combined with DES in the oil matrix,the advantage of which was pointed to rapid and efficient separation of targeted plant growth regulators from complicated oil matrix.The proposed approach achieved satisfied results and met the monitoring requirements of plant growth regulators control in edible vegetable oil samples.