DNA-PK和Cyclin D1在增生性瘢痕成纤维细胞中的表达及与细胞周期相关性的探讨

Expression and correlation to the cell cycle of DNA-PK and Cyclin D1 in hypertrophic scar fibroblasts

目的探讨DNA-PK和Cyclin D1在增生性瘢痕成纤维细胞中的表达及与细胞周期的相关性。方法选取增生性瘢痕患者40例(研究组)及同期行美容手术切取正常皮肤的患者10例(对照组)。研究组按瘢痕形成的不同时间又分为4个亚组,即0~3个月组、4~6个月组、7~12个月组、13~24个月组;每组各10例。分别采用Western blot法和荧光定量PCR法对成纤维细胞不同时间段的细胞周期、DNA-PK和Cyclin D1的蛋白表达以及m RNA表达进行比较。结果就增生性瘢痕成纤维细胞处于G2/M、S期的比例来看,对照组标本与其他4组及4组之间(瘢痕形成0~3个月组、4~6个月组、7~12个月组、13~24个月组)均存在显著的组间差异(P<0.001),0~3个月、4~6个月组>7~12个月、13~24个月组和对照组;DNA-PK和Cyclin D1蛋白表达水平,0~3个月、4~6个月组高于7~12个月、13~24个月组和对照组,其组间比较差异具有统计学意义(P<0.001);DNA-PK和Cyclin D1的m RNA表达水平,0~3个月、4~6个月组高于7~12个月、13~24个月组和对照组,其组间比较差异具有统计学意义(P<0.001)。结论 DNA-PK和Cyclin D1在成纤维细胞中,其蛋白质与m RNA的表达强度逐渐减弱;在增生性瘢痕的早期对DNA-PK和Cyclin D1进行干预,可能会对增生性瘢痕的发生与发展起到控制作用。

Objective To explore the expression and correlation to the cell cycle of DNA-PK and Cyclin D1 in hypertrophic scar fibroblasts. Methods From May 2017 to December 2017, a total of 40 patients with hypertrophic scars were enrolled in the study group.Ten patients who underwent cosmetic surgery to remove normal skin were selected as the control group. The study group was divided into4 subgroups according to the time of scar formation, including a 0 to 3 months group, a 4 to 6 months group, a 7 to 12 months group and a13 to 24 months group, with 10 patients in each group. The cell cycle of fibroblasts, expression of DNA-PK, Cyclin D1 protein and m RNA was compared through Western Blot and the fluorescence quantitative PCR method. Results The number of hypertrophic scar fibroblasts in the G2/M and S phases in the 0 to 3 months and 4 to 6 months groups was more than those in the 7 to 12 months, 13 to 24 months, and control groups(P<0.001). The expression of DNA-PK and Cyclin D1 protein in the 0 to 3 months and 4 to 6 months groups was higher than that in the 7 to 12 months, 13 to 24 months, and control groups(P<0.001). The expression of DNA-PK and Cyclin D1 m RNA in the 0 to 3 months and 4 to 6 months group was higher than that in the 7 to 12 months, 13 to 24 months, and control groups(P<0.001).Conclusion The expression of DNA-PK and Cyclin D1 protein and m RNA was gradually weakened in fibroblasts. Therefore, intervention of DNA-PK and Cyclin D1 in the early stage of hypertrophic scars may control the occurrence and development of hypertrophic scars.