SCN10A rs12632942单核苷酸多态性与结直肠癌化疗奥沙利铂外周神经毒性的相关性

Association of SCN10A single nucleotide polymorphism rs12632942 and oxaliplatin-induced peripheral neuropathy in colorectal cancer patients receiving chemotherapy

目的:探讨SCN10A基因外显子区rs12632942位点多态性与结直肠癌(CRC)患者化疗奥沙利铂外周神经毒性(OXLIPN)的相关性。方法:选取2011年1月至2013年6月广州医科大学附属第二医院、南昌大学第二附属医院、广州市白云区中医医院319例接受含奥沙利铂(OXL)化疗方案的CRC患者(均为中国中南地区汉族)血液标本,常规提取DNA、PCR扩增及分析SCN10A外显子多态位点rs12632942基因型,评估OXLIPN程度。通过单因素卡方检验、Logistic多因素回归分析评估外显子多态rs12632942基因型与OXLIPN的相关性。结果:319例CRC患者rs12632942基因型:AA134例、AG156例、GG29例,rs12632942基因型频率符合哈温平衡(P>0.05)。rs12632942的AG+GG基因型与Ⅱ~Ⅳ度OXLIPN相关(P<0.01),是发生Ⅱ~Ⅳ度OXLIPN的独立危险因素(OR=2.044,95%CI=1.231~3.392,P<0.01)。结论:SCN10A基因外显子区rs12632942AG+GG基因型的CRC患者易感Ⅱ~Ⅳ度OXLIPN。

Objective:To explore the association between single nucleotide polymorphism rs12632942 in SCN10A exon and oxaliplatin-induced peripheral neuropathy(OXLIPN)in colorectal cancer(CRC)patients receiving chemotherapy.Methods:A total of 319 cases of blood samples from CRC patients receiving chemotherapy regimen with Oxaliplatin(OXL)were collected from the Second Affiliated Hospital of Guangzhou Medical University,the Second Affiliated Hospital of Nanchang University,and Guangzhou Baiyun District Hospital of Chinese Medicine during January 2011 and June 2013.DNA was routinely extracted,and PCR amplification was performed to analyze the genotype of rs12632942;and OXLIPN of patients was also evaluated.The association between rs12632942 genotype and OXLIPN was analyzed byχ~2 test and multivariate logistic regression model.Results:The genotypes of rs12632942 of 319 CRC patients:AA of 134 cases,AG of 156 cases and GG of 29 cases;and the genotype distribution of rs12632942 was in accordance with Hardy-Weinberg equiliberum(P>0.05).χ~2 test showed that rs12632942 AG+GG genotype was associated withⅡ-Ⅳdegree OXLIPN(P<0.01).Multivariate logistic regression model showed that rs12632942 AG+GG genotype was an independent risk factor forⅡ-Ⅳdegree OXLIPN(OR=2.044;95%CI=1.231-3.392;P<0.01).Conclusion:Colorectal cancer patients with SCN10A exon polymorphism rs12632942 AG+GG genotype were susceptible toⅡ-Ⅳdegree OXLIPN.