IL-34在巨噬细胞分化中的作用研究

Effects of IL-34 on differentiation of macrophages

目的:探索IL-34与M-CSF在诱导骨髓细胞向巨噬细胞分化以及巨噬细胞表型维持中的异质性作用。方法:小鼠骨髓细胞经不同方案培养后,观察细胞形态并计数;流式分析巨噬细胞比例、分化标记物表达水平和细胞凋亡水平;RT-PCR检测巨噬细胞极化相关基因的表达水平。结果:与M-CSF比较,IL-34诱导巨噬细胞在形态、比例和绝对数差异均无显著统计学意义;分化标记物CD11b和F4/80的平均荧光强度(MFI)显著降低(均P<0.05);极化相关基因IL-1β、IL-10和iNOS的表达水平显著升高(均P<0.05),IL-12β表达降低(均P<0.05)。结论:IL-34可诱导骨髓细胞向巨噬细胞分化,促进巨噬细胞增殖的能力与M-CSF相似;促进巨噬细胞分化相关标记和极化相关基因表达与M-CSF存在显著差异。

Objective: To explore the different effect of IL-34 and M-CSF in induction of bone marrow cells differentiation into macrophages and maintenance of macrophage phenotype. Methods: Bone marrow cells were cultured with different cytokines.The number of macrophages was counted and their morphology was observed under microscope.The proportion of macrophages,expression of differentiation markers and apoptosis were determined by flow cytometry.The expression of polarization-related genes in macrophages was detected by RT-PCR. Results: Compared with the M-CSF group,there was no significant difference in morphology,proportion and absolute number of macrophages in IL-34 group.Mean fluorescence intensity (MFI) of the differentiation markers CD11b and F4/80 was significantly reduced ( P <0.05).The expression of polarization-related genes IL- 1β, IL-10 and iNOS was significantly increased ( P <0.05), while the expression of IL- 12 β was decreased ( P <0.05). Conclusion: IL-34 shares similar effect with M-CSF in induction of differentiation and proliferation of macrophages from bone marrow cells.But IL-34 is different from M-CSF in the expression of differentiation markers and polarization-related genes in macrophages.