摘要
目的:研究黄芪多糖不同给药方式对流感病毒PR8小鼠急性肺损伤的保护作用。方法:实验分为正常对照组、PR8组、治疗给药组、预防给药组、利巴韦林对照组。用甲型流感病毒鼠肺适应株A/PR/8/4制备小鼠流感病毒性肺炎模型,黄芪多糖用不同给药方式干预后,观察小鼠肺指数和肺病理改变,用比色法检测血液和肺组织丙二醛(MDA)水平,超氧化物酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,qPCR检测肺组织TNF-α、IL-6、IL-1β、IFN-α mRNA含量,Western blot检测肺组织凋亡相关蛋白Caspase-3、8、9表达。结果:黄芪多糖治疗和预防给药均能降低肺指数,减轻肺损伤;下调肺组织MDA水平及上调SOD、GSH-Px活性(P<0.05);抑制TNF-α、IFN-α、IL-6、IL-1β的分泌(P<0.05);减少Caspase-3、8、9表达(P<0.05)。结论:黄芪多糖通过降低炎性细胞因子分泌和抑制组织细胞凋亡,缓解甲型流感病毒PR8所致的急性肺损伤。
Objective: To investigate the effect of Astragalus polysaccharides(APS) treatment on influenza virus PR8-induced lung injury and explore its protective effect and mechanism. Methods: The mice were divided into control group,treatment group,prevention group and ribavirin control group.The acute lung injury mode was established by infection of influenza virus strain A/PR/8/4(H1N1),and treated with different administration of APS by intragastric administration.After treatment with APS,pulmonary index and pathological changes of lung tissue were observed.The contents of MDA,GSH-Px and SOD in blood and lung tissues was detected by colorimetric method.The expression of inflammatory cytokines TNF-α,IL-6,IL-1β,IFN-α and Caspase-3,8,9 in lung tissue were detected respectively by qPCR and Western blot. Results: APS treatment group and prevention group could reduce the lung index and the lung tissue lesions,down-regulate the level of MDA,TNF-α,IFN-α,IL-6,IL-1β and increase enzyme activity of SOD,GSH-Px( P <0.05).The expression of Caspase-3,8,9 were significant decreased in treatment group and prevention group compared with PR8 group( P <0.05). Conclusion: The different administration of APS can relieve pathological damage of pneumonia induced by influenza virus PR8 through reducing the levels of inflammatory cytokines and inhibiting cell apoptosis.
作者
卢春化
马艳梅
王红霞
薛凌(指导)
LU Chun-Hua;MA Yan-Mei;WANG Hong-Xia;XUE Ling(First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2019年第14期1699-1702,1707,共5页
Chinese Journal of Immunology
基金
国家重大传染病防治科技重大专项项目(2012ZX10004209-001)
辽宁省自然科学基金项目(20170540372)