植物发育相关miR319基因家族进化及靶基因预测分析

Moleculer Evolution and Targets Prediction of miR319 Gene Family

【目的】研究miR319靶基因的功能和相关生理作用机制。【方法】利用生物信息学的方法对miR319基因家族成员成熟序列进行分析及靶基因预测。【结果】miR319在不同植物中拷贝数差异较大,大部分miR319基因分散在不同染色体上,少部分分散在同一条染色体上;miR319序列在不同物种间具有高度的保守性,保守区位置分布差异较大。进化分析表明miR319基因家族的成员呈现4大分支,水稻osa-miR319a-3p和苹果mdm-miR319c有特殊的进化机制。靶基因预测表明miR319靶基因功能包括转录因子、翻译活化剂、还原酶、蛋白酶等。【结论】miR319靶基因在植物体内可能形成复杂的调控网络对植物生长发育和植物抗逆过程起着重要的调控作用,这为今后进一步研究miR319靶基因的功能和相关生理作用机制提供了重要的理论依据。

【Objective】In this paper, the function and molecular machanism of miR319 target gene were studied.【Method】The mature sequences of miR319 gene family were analyzed and their target genes were predicted by bioinformatics method. 【Result】The results showed that in different plants the copy numbers of miR319 were quite different, most of the miR319 gene were scattered on different chromosomes and a few of them were scattered on the same chromosome.miR319 sequences were highly conserved among different species and the conservative location distribution are quite different.Phylogenetic analysis revealedthat the members of the miR319 gene family showed four major branches, and the osa-miR319 a-3 p and the apple mdm-miR319 c had a special evolutionary mechanism. Target gene prediction indicated that miR319 target gene functions included transcription factors, translation activator,reductase, proteinase etc.【Conclusion】The miR319 target gene may form a complex regulatory network in plants which plays an important role in regulating plant growth and plant resistance.The above results provide an important theoretical foundation for further study of the function and related physiological mechanism of miR319 target gene.