通咽喷雾剂对短暂性大脑中动脉栓塞模型大鼠吞咽功能的干预效应

Influences of Tongyan Spray on Swallowing Function in Rats with Transient Middle Cerebral Artery Occlusion

目的 观察通咽喷雾剂(TYSpray)对短暂性大脑中动脉栓塞(tMCAO)模型大鼠吞咽功能的影响。方法 将大鼠随机分为Sham组、tMCAO组、tMCAO+TYSpray组。tMCAO组采用线栓法阻塞大鼠大脑中动脉,于90min后再灌注,以建立tMCAO大鼠模型。tMCAO+TYSpray组大鼠在造模后给予通咽喷雾剂口咽部喷雾用药,Sham组、模型组给予等量生理盐水。观察大鼠一般营养状况并记录死亡数;于7d、14d时,记录大鼠体重及进食水量;于14d时,观测大鼠吞咽潜伏期及吞咽次数。结果 tMCAO组大鼠除表现为吞咽潜伏期延长和吞咽次数减少等吞咽直接指标的异常,还表现为毛发干枯少泽、活动度减低、体型瘦弱、精神状态不佳、死亡率高、体重下降和体重增量/体重比值下降、进食量减少、进水量减少和进水量/体重比值下降等吞咽间接指标的异常。tMCAO+TYSpray组大鼠未见吞咽潜伏期延长和吞咽次数减少,而表现为吞咽次数明显增加,在进食量/体重比值方面虽与tMCAO组大鼠相比差异无统计学意义,但表现出一定的改善趋势,在14d时的进水量/体重比值与tMCAO组大鼠相比明显增加。结论 通咽喷雾剂可有效改善tMCAO组大鼠的吞咽功能,在改善进食水方面亦显示出较好的改善趋势。

Objective To investigate the effects of Tongyan Spray(TY Spray) on swallowing function of transient middle cerebral artery occlusion (tMCAO) rats. Methods Rats were randomly divided into Sham,tMCAO,tMCAO+TY Spray group.The middle cerebral artery of rats in the tMCAO group was blocked by introducing nylon threads,and then re-infused 90 minutes later to establish the rat model of tMCAO.TY Spray was given to rats in tMCAO+TY Spray group by oropharyngeal Spray after modeling,Sham group and model group were replaced with the same amount of normal saline.General nutritional status of rats was observed and deaths were recorded.At 7 d and 14 d,the rats′ body weight,food intake and water intake were measured.At 14 d,the onset latency and number of swallows were measured. Results Rats in tMCAO group manifested as not only the abnormal swallowing direct indicators containing prolonged onset latency and reduced numbers of swallow,but also manifested as the abnormal indirect indicators containing dry hair,reduced activity,body emaciated,poor mental state,high mortality,weight loss and decreased weight growth rate,reduced food intake,reduced water intake and decreased water intake per body weight.Rats in tMCAO+TY Spray group didn’t shown prolonged onset latency and reduced numbers of swallow,however manifested as significant increase in numbers of swallow.Although there was no statistical difference on the food intake per body weight when compared with rats in tMCAO group,a better improvement trend was shown in tMCAO+TY Spray group.At 14 d,water intake per body weight of rats in tMCAO+TY Spray group was significantly increased when compared with that in tMCAO group. Conclusion Tongyan spray can effectively improve the swallowing function of tMCAO rats,and has a good improvement trend in improving the food and water intake.