GAS5和miR-21在前列腺癌中表达的关系及临床意义

Relationship and clinical significance of GAS5 and miR-21 expression in prostate cancer

目的:分析GAS5和miR-21在前列腺癌中的表达关系,并探讨其临床意义.方法:收集2011年1月至2013年6月在本院行手术切除治疗的80例前列腺癌患者的肿瘤组织和癌旁非肿瘤组织,采用qRT-PCR检测组织中GAS5和miR-21相对表达量,分析肿瘤组织中GAS5和miR-21表达的关系及两者与临床病理特征的关系,采用K-M生存函数分析肿瘤组织中GAS5和miR-21表达与患者预后的关系.结果:肿瘤组织中GAS5相对表达量低于癌旁非肿瘤组织(P<0.05),miR-21相对表达量高于癌旁非肿瘤组织(P<0.05);肿瘤组织中GAS5和miR-21表达水平呈负相关(r=-0.702,P<0.001);肿瘤组织中GAS5和miR-21表达水平与患者TNM分期、Gleason评分和淋巴结未转移有关,与血清PSA水平及前腺体体积无关;80例患者5年总生存率86.25%(69/80),GAS5高表达组无瘤生存期和总生存率均高于GAS5低表达组(P<0.05),miR-21高表达组无瘤生存期和总生存率均低于miR-21低表达组(P<0.05);TNM分期、Gleason评分、淋巴结转移、血清PSA水平及GAS5表达水平、miR-21表达水平是影响前列腺癌患者预后的独立危险因素.结论:前列腺癌中GAS5表达降低,miR-21表达升高,与患者不良预后有关,提示GAS5和miR-21可作为前列腺癌的预后标志物.同时GAS5和miR-21呈负相关,提示两者可能在调控前列腺癌进展及不良预后中存在相互作用.

Objective:To analyze the expressions of GAS5 and miR-21 in prostate cancer and explore their clinical significances. Methods:Tumor tissues and adjacent non-tumor tissues were collected from 80 patients with prostate cancer underwent surgical resection in our hospital from January 2011 to June 2013. The relative expressions of GAS5 and miR-21 in tissues were detected by qRT-PCR, analyzed the relationship between the expressions of GAS5 and miR-21 in tumor tissues and the clinicopathological characteristics of patients. K-M survival function was used to analyze the relationships between the expressions of GAS5 and miR-21 with the prognosis of patients. Results:The relative expression of GAS5 in cancer tissues was lower than that in noncancer tissues adjacent to cancer (P<0.05), and the relative expression of miR-21 was higher than that in noncancer tissue adjacent to cancer (P<0.05);there was a negative correlation between the expressions of GAS5 and miR-21 (r=?0.702, P<0.001);the expression levels of GAS5 and miR-21 in tumors were correlated with TNM stage, Gleason score, and lymph node metastasis, but not with serum PSA level and anterior gland volume;the 5-year overall survival rate of 80 patients was 86.25%(69/80), the tumor-free survival and overall survival rate of patients with GAS5 high expression were higher than those with GAS5 low expression (P<0.05), the tumorfree survival and overall survival rate of patients with miR-21 high expression were higher than those with miR- 21 low expression (P<0.05);TNM staging, Gleason score, lymph node metastasis, serum PSA level, and GAS5 expression level, and miR-21 expression level were independent risk factors affecting the prognosis of prostate cancer patients. Conclusion:The expression of GAS5 is decreased and the expression of miR-21 is increased in prostate cancer, which is related to the poor prognosis of the patients, these results suggest that GAS5 and miR-21 can be used as prognostic markers of prostate cancer. At the same time, GAS5 is negatively correlated with miR- 21, these results suggest that there may be an interaction between them in regulating the progression and adverse prognosis of prostate cancer.