槐米提取物对类风湿关节炎大鼠Th17/Tr eg细胞平衡的调节作用

Regulation of Sophora japonica extract on Th17/Treg cell balance in rheumatoid arthritis rats

目的探讨槐米提取物对类风湿关节炎大鼠辅助性T细胞/调节性T细胞(Th17/Treg)细胞平衡调节作用。方法将50只雄性SD大鼠适应性饲养7d后,选取合格大鼠,并将其随机分为正常组、模型组、槐米组,每组15只。建立Ⅱ型胶原诱导性关节炎大鼠模型,槐米组于造模第21天开始灌胃给予槐米提取液300mg/(kg·d),1次/d。治疗4周后,采用酶联免疫吸附测定法检测各组血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、IL-10、IL-17、IL-21、IL-23、IL-27水平,采用流式细胞术检测外周血中Th17/Treg的比率,通过病理切片观察踝关节病理学变化。结果与模型组比较,槐米组大鼠血清中TNF-α、IL-6、IL-17、IL-21、IL-23含量明显下降,IL-10、IL-27水平明显上调,外周血中Th17/Treg比率显著降低,差异有高度统计学意义(P<0.01)。结论槐米能够通过调控相关免疫细胞因子水平,诱导Treg细胞生成的同时干预Th17细胞分化,改善Th17/Treg失衡状态,从而起到治疗类风湿关节炎的作用。

Objective To investigate the effect of Sophora japonica extract on the balance of helper T cells/regulatory T cells (Th17/Treg) in rheumatoid arthritis rats.Methods Fifty male SD rats were fed adaptively for 7 days.Qualified rats were selected and randomly divided into normal group,model group and Sophora japonica group,with 15 rats in each group.A rat model of type Ⅱ collagen induced arthritis was established.Sophora japonica group was given 300 mg/(kg·d) of Sophora japonica extract by gastric perfusion on the 21st day of model establishment,once a day.After 4 weeks of treatment,serum levels of tumor necrosis factor-α(TNF-α),interleukin (IL)-6,IL-10,IL-17,IL-21,IL-23 and IL-27 were measured by ELISA.The ratio of Th17/Treg in peripheral blood was detected by flow cytometry.The pathological changes of ankle joint were observed by pathological section.Results Compared with the model group,the levels of TNF-α,IL-6,IL-17,IL-21 and IL-23 in the serum of rats in Sophora japonica group decreased significantly,the levels of IL-10 and IL-27 increased significantly,and the ratio of Th17/Treg in peripheral blood decreased significantly (P < 0.01).Conclusion Sophora japonica can induce cytopoiesis of Tregs while interfering with the differentiation of Th17 by regulating the related immunocytokines,which improves Th17/Treg imbalance,thus exerting therapeutic effect for rheumatoid arthritis.