急性髓系白血病患者血清sPD-L1、VEGF表达及相关性

Expression and correlation of serum sPD-L1 and VEGF in patients with acute myeloid leukemia

目的探讨急性髓系白血病患者血清可溶性程序性死亡因子配体-1(sPD-L1)、血管内皮生长因子(VEGF)的表达及相关性。方法选择2015年1月~2018年1月广东省东莞康华医院(以下简称“我院”)收治的89例急性髓系白血病患者为研究对象,纳入观察组,另选取我院同期进行健康体检的健康受试者50名为对照组。采用酶联免疫吸附法(ELISA)检测两组受试者的sPD-L1、VEGF水平,并进行组间比较。对观察组患者进行2个月标准化治疗,根据疗效判断标准将患者分为完全缓解(CR)组36例、部分缓解(PR)组40例和未缓解(PD)组13例,对三组患者的sPD-L1、VEGF水平进行检测比较,并对sPD-L1与VEGF的相关性进行分析探讨。结果治疗前,观察组患者sPD-L1、VEGF水平均显著高于对照组,差异有统计学意义(P<0.05)。观察组经治疗后,CR组和PR组患者sPD-L1、VEGF水平均显著低于PD组,且CR组患者sPD-L1、VEGF水平低于PR组,差异有统计学意义(P<0.05)。经Pearson相关性检验分析,患者治疗前、治疗后血清sPD-L1与VEGF均呈显著的正相关性(r=0.447、0.327,均P<0.05)。结论急性髓系白血病患者sPD-L1、VEGF呈现高表达,随着预后效果的好转,其表达水平不断降低。因此,sPD-L1、VEGF水平的检测对于急性髓系白血病的诊治和预后评估有一定辅助作用和临床价值。

Objective To investigate the expression and correlation of serum soluble programmed death ligand 1 (sPD-L1) and vascular endothelial growth factor (VEGF) of patients with acute myeloid leukemia.Methods Eighty-nine patients with acute myeloid leukemia admitted to Dongguan Kanghua Hospital (“our hospital” for short) from January 2015 to January 2018 were selected as research objects and enrolled into observation group,and 50 healthy subjects who underwent health examination in our hospital during the same period were selected as the control group.The sPD-L1 and VEGF levels of the two groups were detected by enzyme-linked immunosorbent assay (ELISA) and compared between the two groups.The patients in the observation group were treated with standardized therapy for 2 months,according to the criteria of curative effect,the patients were divided into complete remission (CR) group (36 cases),partial remission (PR) group (40 cases) and proceed (PD) group (13 cases),and the levels of sPD-L1 and VEGF in the three groups were detected and compared.The correlation between sPD-L1 and VEGF was also analyzed.Results Before treatment,the levels of sPD-L1 and VEGF in the observation group were significantly higher than those in the control group,the differences were statistically significant (P < 0.05).After treatment,the levels of sPD-L1 and VEGF in CR group and PR group were significantly lower than those in PD group,and the levels of sPD-L1 and VEGF in CR group were lower than those in PR group,the differences were statistically significant (P < 0.05).Pearson correlation test showed that sPD-L1 was positively correlated with VEGF (r = 0.447,0.327,all P < 0.05).Conclusion The expression of sPD-L1 and VEGF in acute myeloid leukemia patients is high,and the levels of sPD-L1 and VEGF are decreased with the improvement of prognosis.Therefore,the detection of sPD-L1 and VEGF has certain assistant effects and clinical value for the diagnosis,treatment and prognosis evaluation of acute myeloid leukemia patients.