膀胱癌组织中UCA1、miR-141的表达及临床意义

Expression and clinical significance of UCA1 and miR-141 in bladder cancer tissue

目的研究膀胱癌组织中尿路上皮癌抗原1(UCA1)、micoRNA-141(miR-141)的表达及临床意义。方法以2013年2月~2015年2月山东省临沂市肿瘤医院收治的膀胱癌患者83例为研究对象。采用实时荧光定量聚合酶链式反应检测UCA1、miR-141表达情况,并分析UCA1、miR-141表达与患者临床病理特征的关系。此外,对所有患者均进行为期3年的随访,将其按照生存状况的不同分为存活组(n=39)与死亡组(n=44),比较两组UCA1、miR-141表达情况。结果膀胱癌组织中UCA1、miR-141阳性率相比癌旁正常组织较高(均P<0.05)。病理分级为G1、临床分期T1~T2期、无淋巴结转移的膀胱癌组织中UCA1阳性率相比病理分级为G2~G3、临床分期T3~T4期、有淋巴结转移较低(均P<0.05)。临床分期T1~T2期、无淋巴结转移的膀胱癌组织中miR-141阳性率相比临床分期T3~T4期、有淋巴结转移较低(均P<0.05)。存活组膀胱癌组织中UCA1、miR-141阳性率相比死亡组较低(均P<0.05)。结论膀胱癌组织中UCA1、miR-141均存在明显高表达,临床工作中可通过上述两项指标的检测进行膀胱癌患者病情判断以及预后评估。

Objective To study the expression and clinical significance of urothelial carcinoma antigen 1 (UCA1) and micoRNA-141 (miR-141) in bladder cancer tissue.Methods A total of 83 patients with bladder cancer admitted to Linyi Cancer Hospital from February 2013 to February 2015 were taken as research objects.The expression of UCA1 and miR-141 were detectd by real-time fluorescent quantitative polymerase chain reaction and the relationship between the expression of UCA1 and miR-141 and the clinicopathological characteristics of the patients was analyzed.In addition,all patients were followed up for 3 years and divided into survival group (n = 39) and death group (n = 44) according to different survival conditions.The expression of UCA1 and miR-141 was compared between the two groups.Results The positive rate of UCA1 and miR-141 in bladder cancer tissues was higher than those in adjacent normal tissues (all P < 0.05).The positive rate of UCA1 in bladder cancer with pathological grade G1,clinical stage T1-T2 and no lymph node metastasis was lower than that in bladder cancer with pathological grade G2-G3,clinical stage T3-T4 and lymph node metastasis (all P < 0.05).The positive rate of miR-141 in bladder cancer tissues with clinical stage T1-T2 and no lymph node metastasis was lower than that in bladder cancer tissues with clinical stage T3-T4 and lymph node metastasis (all P < 0.05).The positive rate of UCA1 and miR-141 in survival group was lower than those in death group (all P < 0.05).Conclusion UCA1 and miR-141 is highly expressed in bladder cancer tissues.The two indicators mentioned above can be used in clinical work to evaluate the condition and prognosis of patients with bladder cancer.