肝癌不同靶标核酸适配体的筛选研究进展

Research progress in screening aptamers of different targets on liver cancer

早发现、早治疗是改善肝癌患者预后和降低病死率的关键。筛选具有高灵敏度和高特异性的分子识别探针对肝癌的早期诊断、预警、转移和疗效评估等具有重要意义。利用体外指数富集配体系统进化技术(systematic evolution of ligands by exponential enrichment,SELEX)筛选获得的核酸适配体,能够高特异性、高亲和力地与多种靶标(包括小分子、蛋白质、细胞、组织、微生物等)结合,为肝癌的特异识别、早期诊断、病理分型提供了有效方法。本研究主要阐述以肝癌细胞系、肝癌血清、临床肝癌组织为靶标的核酸适配体的筛选研究进展。

The early detection and treatment is key way to improving the prognosis and reducing mortality of patients with liver cancer.The molecular recognition probes with high sensitivity and specificity was selected that has significantly value on the early diagnosis,early warning,metastasis and therapeutic evaluation of liver cancer.Aptamers were screened through systematic evolution of ligands by exponential enrichment(SELEX),which were combined with a variety of targets(including small molecules,proteins,cells,tissues,microorganisms,etc.)with high specificity and affinity,and it is an important method to screen valued targets for specific identification,early diagnosis and pathological classification of liver cancer.This study reviewed the progress in screening of different target aptamers about cell lines,serum and clinical cancer tissues of liver cancer.