利用SSR分析浙南地区甜玉米自交系的遗传多样性

Genetic diversity analysis of sweet maize inbred lines in Southern Zhejiang by SSR markers

为评价浙南地区甜玉米自交系的遗传多样性,指导甜玉米新品种的选育,本研究利用SSR分子标记对37份浙南地区甜玉米自交系进行了分析。结果显示,37个多态性SSR标记共检测到209个等位基因变异,平均为5.65个。SSR标记的多态性信息量PIC值为0.62~0.95,平均为0.82。自交系间的相似性系数为0.12~0.94,平均为0.40,说明这些甜玉米自交系之间亲缘关系较远,具有丰富的遗传多样性。UPGMA聚类分析将37份甜玉米自交系划分为3个类群,划分结果与其系谱及地理分布基本吻合。

To evaluate the genetic diversity of sweet maize inbred lines and to guide new sweet maize varieties breeding, 37 sweet maize inbred lines from Southern Zhejiang were analyzed using SSR molecular markers. The results showed that 209 alleles were detected by 37 polymorphic SSR markers, with an average of 5.65 alleles per SSR marker. Polymorphism information content (PIC) values of SSR markers were 0.62-0.95, with an average of 0.82 per primer pair. Similarity coefficients among inbred lines ranged from 0.12 to 0.94, with an average of 0.40, which suggested these sweet maize inbred lines had abundant genetic diversity. The 37 sweet maize inbred lines were classified into three groups by UPGMA method, which was consistent with the pedigree analysis and geographical distribution.