饲料磷真消化率不同的山羊空肠菌群结构差异研究

Differences in jejunum microbes of goats with different phosphorus true digestibility

动物对饲料磷消化率的提高对于降低养殖成本和减少环境污染均具有重要意义。通过高通量测序技术比较饲料磷真消化率不同的山羊空肠微生物结构的差异。本研究选取24只10月龄,雌性,平均体质量(24.25±2.47)kg的努比亚黑山羊作为试验动物,通过代谢试验筛选出高(HP)、低(LP)饲料磷真消化率组,每组各6只。分别采集HP、LP组山羊空肠内容物,提取微生物总DNA,利用高通量测序平台对细菌16S rRNA基因的高可变区进行测序,使用QIIME等软件对测序数据进行生物信息学分析。研究表明,在门水平,厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)在两组山羊空肠微生物中均为优势菌门,其中HP组厚壁菌门(Firmicutes)的相对丰度极显著低于LP组( P <0.01),而拟杆菌门(Bacteroidetes)的相对丰度极显著高于LP组( P <0.01);在属水平上,HP组 Prevotella 、 Fibrobacter 、 Ruminococcus _2和 Clostridium_sensu_stricto _1的相对丰度显著高于LP组( P <0.05),而 Desulfovibrio 和 Victivallis 的相对丰度显著低于LP组( P <0.05)。饲料磷真消化率不同的山羊空肠菌群结构存在显著差异。

Improving animal digestion and utilization efficiency of phosphorus were of great significance for reducing animal breeding costs and environmental pollution. The objective of this study was to compare the structure of jejunum microorganism of goats with different true digestibility of feed phosphorus using Illumina MiSeq sequencing technology. Twenty-four Nubian black goats, aged 10 months on average and weighed (24.25±2.47)kg, were selected as the experimental animals. The contents of jejunum of each goat in the HP and LP groups were collected and total genomic DNA was extracted, and the high variable region of 16S rRNA gene of bacteria was sequenced by high throughput sequencing platform. Bioinformatics analysis of sequencing data was carried out using QIIME software. At the phylum level, Firmicutes and Bacteroidetes were the dominant bacteria of the jejunum microbiota in goats, and the relative abundance of Firmicutes in HP group was extremely significantly lower than that in the LP group ( P <0.01), while the relative abundance of Bacteroidetes was extremely significantly higher than that in the LP group( P < 0.01). At the genus level, the relative abundances of Prevotella, Rikenellaceae _RC9_gut_group, Fibrobacter, Ruminococcus _2 and Clostridium_sensu_stricto_ 1 in HP group were significantly higher than that in the LP group ( P < 0.05), and the relative abundances of Desulfovibrio and Victivallis were significantly lower than that in LP group( P <0.05). There were significant differences in structure of jejunum microorganisms among goats with different true digestibility of feed phosphorus.