大黄复方膏外敷预防留置针静脉治疗所致机械性静脉炎的实验研究

Experimental Study on Prevention of Mechanical Phlebitis Caused by Indwelling Needle Intravenous Therapy with Rhubarb Compound Ointment

目的:探究大黄复方膏外敷预防留置针静脉治疗所致机械性静脉炎的效果。方法:选取同种健康新西兰大耳兔56只,采用数字表法随机分组,8只作为空白对照组,其余48只分为对照组和实验组各24只,实验组和对照组均在兔双耳外缘行留置针静脉穿刺置管,每日按时输注生理盐水20ml,对照组置管后不做任何预防处理,实验组则采用大黄复方膏于穿刺部位外敷,保持药物接触6h/d,置管7天后拔除留置针,取组织标本镜下观察评价炎症反应情况,并检测血清肿瘤坏死因子-α(TNF-α)、白介素-1(IL-1)、白介素-6(IL-6)水平进行对比。结果:实验组16只未见炎症反应,6只轻度炎症反应,2只中度炎症反应,对照组4只未见炎症反应,11只轻度炎症反应,7只中度炎症反应,2只重度炎症反应,两组对比差异具有统计学意义(x2=7.633,P<0.05);实验组IL-6水平显著高于空白组(P<0.05),但TNF-α、IL-1水平与空白组相比无统计学意义(P>0.05),对照组TNF-α、IL-1、IL-6水平均显著高于实验组及空白组,差异具有统计学意义(P<0.05)。结论:大黄复方膏外敷预防留置针静脉治疗所致机械性静脉炎的效果显著,可有效减轻组织炎症反应程度,降低血清炎症因子含量。

Objective: To investigate the effect of rhubarb compound ointment on prevention of mechanical phlebitis caused by indwelling needle intravenous therapy. Methods: 56 healthy New Zealand rabbits with selected randomly 8 groups, as control group, the remaining 48 were divided into control group and experimental group with 24 rats in each group, the experimental group and the control group were retained in the rabbit ears for the outer needle vein puncture, infusion of normal saline daily on time 20ml, the control group after the catheter without any preventive treatment, the experimental group received Compound Rhubarb cream on the puncture site for maintaining drug contact 6h/d, 7d catheter after removal of indwelling needle, evaluate the condition of inflammation tissue specimens under the microscope, and the detection of serum tumor necrosis factor alpha(TNF-alpha), interleukin-1(IL-1), interleukin-6 (IL-6) level were compared. Results: the experimental group 16 no inflammation, 6 mild inflammation, 2 moderate inflammatory reaction, 4 rats in the control group no inflammation, 11 mild inflammation, 7 moderate inflammation, 2 severe inflammatory reaction, with significant difference between the two groups ( x 2 =7.633, P <0.05);level IL-6 experimental group was significantly higher than that of control group ( P <0.05), but compared to TNF- alpha, IL-1 level and blank group had no statistical significance ( P >0.05), the control group of TNF-alpha and IL-1 and IL-6 were significantly higher than the experimental group and the control group, the difference was statistically significant ( P <0.05). Conclusion: Rhubarb compound ointment is effective in the prevention of mechanical phlebitis caused by indwelling needle intravenous therapy, which can effectively reduce the degree of inflammation and reduce the level of serum inflammatory factors.