水稻新型胞质不育系L-orfH79与其同核异质YTA的比较

Comparison of New Cytoplasmic Sterile Lines L-orfH79 and Its Isonuclear Alloplasmic Sterile Lines YTA of Rice

[目的]验证新型细胞质不育系L- orfH79 与其同核异质的近等基因系HL-CMS YTA的区别。[方法]用 orfH79和orf290 这2种分子标记对L- orfH79 和YTA进行分子鉴定,并比较其花粉染色特性、花粉育性、结实率及 orfH79 表达量。[结果]分子标记鉴定结果显示L- orfH79只含有不育基因orfH79、不含有orf290 ,YTA同时含有 orfH79和orf290。 L- orfH79 花粉的淀粉沉积比YTA更少,L- orfH79 花粉大小均匀,而YTA的花粉形态不一。L- orfH79 花粉育性、套袋结实率及自然结实率(都低于10%)略高于YTA(都低于5%)。当L- orfH79中引入恢复基因Rf5或者Rf6后花粉形态和颜色都恢复正常。抽穗期 L- orfH79剑叶中的 orfH79 表达量低于YTA,在L- orfH79中引入Rf5或者Rf6后会减少orfH79的表达量。[结论] L- orfH79 是不同于YTA的新型细胞质雄性不育系,L- Rf5 、L- Rf6 可作为其恢复系。

[Objective]In order to verify the difference between the new cytoplasmic male sterile line L- orfH79 and its isonuclear alloplasmic sterile lines HL-CMS YTA.[Method]L- orfH79 and YTA were identified by molecular markers orfH79 and orf290 , of which pollen dyeing characteristics, pollen fertility, seed setting rate and expression level of orfH79 were compared.[Result]It showed that both orfH79 and orf290 in YTA, while L- orfH79 only contained orfH79 but not orf290 by molecular marker identification. The starch deposition of L- orfH79 pollen was less than that of YTA. The size of L- orfH79 pollen was uniform, but the morphology of YTA pollen was different. The pollen fertility, bagging seed setting rate and natural seed setting rate of L- orfH79 (all below 10%) were slightly higher than YTA (all below 5%). Pollen morphology and color returned to normal when the restorer gene Rf5 or Rf6 was introduced into L- orfH79 . The expression of orfH79 in flag leaves of L- orfH79 was lower than that of YTA. The expression of orfH79 reduced in sterile line L- orfH79 after introducing Rf5 or Rf6 .[Conclusion] L- orfH79 is a new cytoplasmic male sterile line that different from YTA. L- Rf5 and L- Rf6 can be used as restorer lines.