荷瘤小鼠MDSCs中TRIM25和PTEN的表达

Expression of TRIM25 and PTEN in MDSCs of tumor-bearing mice

目的:探讨荷瘤小鼠肿瘤组织来源的髓源抑制性细胞(myeloid derived suppressor cells,MDSCs)中三基序蛋白25(tripartite motif containing 25,TRIM25)和人第10号染色体缺失的磷酸酶及张力蛋白同源基因(phosphatase and tensin homologue deleted on chromosome ten,PTEN)的表达水平及其意义。方法:构建小鼠CT26结肠癌移植瘤模型,采用实时荧光定量PCR(real time fluorescene quantitative PCR,qRT-PCR)技术检测荷瘤小鼠肿瘤组织来源MDSCs和野生型小鼠脾脏来源MDSCs中TRIM25、PTEN的表达情况。结果:在小鼠CT26结肠癌移植瘤模型中,与野生型小鼠脾脏来源的MDSCs相比,荷瘤小鼠肿瘤组织来源MDSCs中TRIM25表达水平明显升高(P <0. 001),PTEN表达水平显著下降(P <0. 001)。结论:荷瘤小鼠肿瘤组织来源MDSCs中TRIM25的表达升高,PTEN表达下降,提示两者或许参与调控MDSCs。

Objective: The study was to investigate the expression and significance of TRIM25 and PTEN in myeloid-derived suppressor cells( MDSCs) from tumor tissue of tumor-bearing( TB) mice.Methods: CT26 colon cancer tumor-bearing mice models were established. The expressions of TRIM25 and PTEN were detected in MDSCs derived from tumor tissue of TB mice and spleen of wild-type( WT)mice with qRT-PCR method. Results: In CT26 cells bearing mice models,the expression level of TRIM25 in tumor tissue-derived MDSCs of TB mice was significantly higher than that from spleen of WT mice( P < 0. 001). Inversely,compared to WT mice,PTEN expression was obviously down-regulated in MDSCs isolated from tumor tissue of TB mice( P < 0. 001). Conclusion: TRIM25 expression level was up-regulated,while the expression of PTEN was suppressed in MDSCs from tumor tissue of TB mice.They both may participate in the regulation of MDSCs.