IL-1β和IGF-1在幼兔发育性髋脱位髋臼软骨细胞中的表达和作用

Expression and role of IL-1β and IGF-1 in acetabular chondrocytes with developmental dislocation of the hip in immature rabbits

目的探讨白细胞介素-1β(IL-1β)和胰岛素样生长因子-1(IGF-1)在发育性髋脱位(DDH)髋臼软骨细胞增殖和凋亡中的作用及意义。方法选取4周龄实验幼兔32只,采用左侧后肢伸膝屈髋位管型石膏固定方法制作DDH动物模型,右侧后肢不做特殊处理作为对照侧,将幼兔平均分为4组(A、B、C、D组),分别在石膏固定8、12、16、20周后处死,采用免疫组织化学法和Western blot法检测双侧髋臼外缘软骨块及细胞中IL-1β和IGF-1的表达情况和蛋白水平,结果进行统计学分析。结果IL-1β和IGF-1在双侧髋臼软骨细胞中均有不同程度的阳性表达;D组实验侧髋臼软骨中IL-1β的阳性率和蛋白水平最高,B、C、D组实验侧和对照侧比较,差异有统计学意义(P<0.05)。C组实验侧髋臼软骨中IGF-1的阳性率最高,B组实验侧髋臼软骨中IGF-1的蛋白水平最高,B、C组实验侧和对照侧比较,差异有统计学意义(P<0.05)。结论IL-1β与IGF-1在DDH髋臼软骨细胞中的表达异常,导致软骨细胞增殖和凋亡之间失衡,可能是DDH发生髋关节退行性变的原因之一。

Objective To explore the role and significance of interleukin-1beta (IL-1β) and insulin-like growth factor-1 (IGF-1) in the proliferation and apoptosis of acetabular chondrocytes of developmental dislocation of the hip (DDH). Methods Thirty-two experimental rabbits aged 4 weeks old were selected,and the DDH animal model was made by plaster fixation in the knee extension and hip flexion position of the left hind limb.The right hind limb was not treated as a control side,and the rabbits were divided into four groups (group A,B,C and D) were sacrificed after 8,12,16 and 20 weeks of gypsum fixation.Immunohistochemistry and Western blot were used to detect IL-1β and IGF-1 in bilateral acetabular rim cartilage blocks.The expression and protein levels were analyzed and the results were statistically analyzed. Results IL-1β and IGF-1 were positively expressed in bilateral acetabular chondrocytes.The positive rate and protein content of IL-1β in experimental acetabular chondrocytes in group D were the highest.Compared with the control side,the difference among group B,C and D was statistically significant ( P <0.05).The positive rate of IGF-1 in experimental acetabular cartilage in group C was the highest,while the protein content of that was the highest in group B.Compared with the control side,the difference between B and C was statistically significant ( P <0.05). Conclusion The abnormal expression of IL-1β and IGF-1 leads to the imbalance between proliferation and apoptosis of acetabular chondrocytes in DDH,which may be one of the causes of hip degeneration resulted by DDH.