菊芋全粉对刺参生长性能及灿烂弧菌刺激下免疫因子应答变化的影响

Effects of Jerusalem artichoke powder on growth and immune responses after Vibrio splendidus infection in sea cucumber Apostichopus japonicus

为研究菊芋全粉对刺参生长性能及灿烂弧菌刺激下体液中免疫因子活力变化的影响,试验选取初始体重为(4.7±0.1)g的刺参360只,随机分为3组。对照组喂食基础日粮,试验Ⅰ、Ⅱ组分别在基础日粮中添加菊芋全粉5、15g/kg,试验期为42d,分为养殖试验期(0~28d)和感染试验期(29~42d)。试验结果表明:(1)养殖试验期,相较于对照组,菊芋全粉处理组刺参的日增重无显著变化(P>0.05),但试验Ⅰ组(5g/kg)刺参的饲料利用率降低0.75%,试验Ⅱ组(15g/kg)刺参的饲料利用率提高了21.05%(P<0.05);(2)感染试验期,灿烂弧感刺激后,15g/kg菊芋全粉可显著改善酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)的免疫应答变化(P<0.05);而相较于对照组,5g/kg菊芋全粉可显著增强刺激前期刺参的溶菌酶(LYZ)活力(P<0.05)。综上,灿烂弧菌刺激下,15g/kg菊芋全粉可显著增强刺参免疫因子活力。

The experiment was conducted to study the effects of Jerusalem artichoke powder on the growth performance of Apostichopus japonicus and variation of immune-related enzyme activities after Vibro Splendidus infection.Three hundred and sixty sea cucumbers with initial body weight(4.7±0.1)g were randomly divided into three treatments.Control group was fed basal diet while treatment groups were fed basal diet supplemented with 5,15 g/kg Jerusalem artichoke powder for 42 d.The farming stage was 0 ~ 28 d while the infection stage was 29 ~ 42 d.The results showed as follows:(1) Compared with control group,the feed conversion ratio(FCR) of group Ⅱ was significantly increased by 21.05%(P < 0.05),while the FCR of group Ⅰ was decreased by 0.75%. Additionally,no significant difference was observed in the average day gain(ADG)parameter among different groups(P > 0.05).(2) Supplementation of 15 g/kg Jerusalem artichoke powder significantly improved the activities of acid phosphatase(ACP),alkaline phosphatase(AKP) and superoxide dismutase(SOD) after Vibrio splendidus infection(P < 0.05).In addition,5 g/kg Jerusalem artichoke powder significantly improved the activity of lysozyme(LYZ)(P < 0.05) at the early days of bacterial infection.According to the results,15 g/kg Jerusalem artichoke powder significantly improved the activation of four immune enzymes after Vibrio splendidus infection.