不同采收时期金银花中化学成分及其相关酶活性分析

Chemical Components and Relevant Enzyme Activity of Lonicera japonica at Different Harvest Stages

为进一步探讨金银花活性成分动态变化规律,本研究测定了不同时期金银花及叶中的总酚、类黄酮、绿原酸等活性成分的含量以及苯丙氨酸解氨酶(PAL)、肉桂酸4-羟基化酶(C4H)、多酚氧化酶(PPO)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性,并进行相关性分析。从幼蕾期到金花期,金银花中总酚、类黄酮的含量呈逐渐下降趋势,幼蕾期有最高值,分别为4.42、13.29 mg/g;绿原酸在三青期达到最高值,为33.37 mg/g。叶中的总酚、类黄酮含量呈现波动变化趋势;绿原酸呈现逐渐下降规律,含量与花中相差不多。相关性分析得出金银花中的C4H、PPO、POD与活性物质含量呈正相关关系。花和关联叶中的活性成分含量相差不大,可进一步开发叶子的利用价值。

To further explore the dynamic change rule of active components in Lonicera japonica, we measured the contents of total phenol, total flavonoids, chlorogenic acid and the enzyme activities of phenylalanine ammonia lyase(PAL), cinnamic acid 4-hydroxylase(C4H), polyphenol oxidase(PPO), peroxidase(POD),superoxide dismutase(SOD) in L. japonica at different growth stages. And the correlation analysis was conducted. From bud stage to golden-flower stage, the contents of total phenol and flavonoids in L. japonica showed a trend of gradual decline, and the peak value was 4.42, 13.29 mg/g at bud stage respectively;chlorogenic acid reached the maximum at green bud stage, 33.37 mg/g. The contents of total phenol and flavonoids in leaves showed a fluctuation trend. Chlorogenic acid gradually declined, and the content was similar with that in flowers. The activity of C4H, PPO and POD had positive correlation with the content of active components. The active components in flowers and leaves are similar, so we can further develop the use value of leaves.