一款胚芽为核心的八宝粥的营养成分分析及体外活性评价

Nutrient Ingredients Analysis and in Vitro Activity Evaluation on A Mixed Germ Congee

研究了一款以胚芽为核心的八宝粥的营养成分和抗氧化性能力,以及胚芽球蛋白对RAW264.7巨噬细胞的增殖作用。首先测定了此款八宝粥的各种氨基酸含量,重点考察了γ-氨基丁酸(GABA)的含量;其次分别采用还原Fe^3+、1,1-二苯基-2-三硝基苯肼(DPPH)法、邻苯三酚自氧化法和Folin-Ciocalteu法测定了该产品的总还原力、DPPH自由基清除能力、超氧阴离子自由基清除能力和总多酚的含量;最后分离提取出胚芽球蛋白,并且通过细胞实验分析球蛋白对巨噬细胞的增殖能力。结果表明:该产品氨基酸含量丰富,尤其是GABA含量比较突出,该产品具有很好的抗氧化能力;巨噬细胞的增殖实验表明,该产品具有提高机体的免疫能力的潜力。

【Objective】To analyze nutrient ingredients and the antioxidant capacity of a kind of mixed congee with germ,and study the proliferative effect of the germ globulin to the macrophage RAW 264.7 in vitro.【Method】First of all,the amino acid contents in this product was measured,and theγ-aminobutyric acid(GABA)was investigated among all key materials,the congee and other contrastive products.Secondly,the antioxidant activities of this congee and related substrates was determined by Fe 3+reducing power,the scavenging ability of DPPH free radical,the eliminating capacity of superoxide anion radical and the contents of total polyphenols.What s more,the proliferative activity of the germ globulin to the macrophage RAW 264.7 was determined via cells experiment in vitro.【Result】The germ congee was not only a kind of nutrition-rich food,which was packed with amino acids and GABA,but also had the potential to boost the body immunity.【Conclusion】This work is worth to continue to study further.