摘要
二倍体长穗偃麦草含有抗条锈病、抗赤霉病和耐盐碱等优异基因,是小麦遗传改良的重要基因源之一。为明确1E^e染色体片段对小麦农艺和品质性状的影响,利用1E^e(1A)代换系和中国春为试验材料,多年多点鉴定,农艺性状分析结果表明,1E^e取代1A染色体,不仅降低了旗叶长度和旗叶宽度等农艺性状,而且降低了粒长、粒宽、穗粒数、小穗数和千粒重等产量相关性状,但显著增加了穗长。品质相关性状分析结果表明,1E^e染色体可以显著增加面团最大峰值高度和8分钟带宽,但对蛋白质含量、SDS沉降值、湿面筋含量、面筋指数和峰值高度时间等5个指标上没有显著影响。另外,本研究开发了17个1E^e染色体特异分子标记。总之,1E^e染色体可提高小麦品质,但对产量相关性状有不利影响,本研究开发的分子标记对于进一步打破连锁累赘,创制小麦-长穗偃麦草Glu-Ee1短片段易位系具有重要意义。
Thinopyrum elongatum(Host)D.R.Dewey as one of relatives of wheat,harboring genes resistance to strip rust and Fusarium head blight and tolerance to salinity for wheat improvement,could be used for wheat genetic improvement.To understand the effects of chromosome 1E^e on wheat agronomical and qualityrelated traits,wheat-Th.elongatum(Host)D.R.Dewey chromosome disomic substitution line 1E^e(1 A)from common wheat cultivar Chinese spring was analyzed in multiple environments.In relative to the Chinese Spring,the substitution line showed the reduction of the length and width of wheat flag leaf,the length and width of kernels,no.of spikelets per spike,no.of kernels per spike and thousand kernel weight.However,the length of spikes(P<0.05)was significantly increased.Regarding to the quality-related traits,the substitution line displayed the increases of the dough max peak height and band width of 8 min,but no differences of the grain protein content,SDS sedimentation,wet gluten content,gluten index and peak height time were observed.In addition,17 COS markers specific to chromosome 1E^e were developed in this study.All in all,chromosome 1E^e can improve wheat grain quality traits,but it has negative effects on yield related traits.And the developed markers specific for chromosome 1E^e might be used for developing wheat-Thinopyrum chromosome short segment lines carrying GluEe1 by reducing the chromosomal linkage drag.
作者
郭军
卢明娇
武智民
李豪圣
宫文萍
王灿国
程敦公
刘爱峰
曹新有
刘成
翟胜男
杨足君
刘建军
孔令让
赵振东
宋健民
GUO Jun;LU Ming-jiao;WU Zhi-min;LI Hao-sheng;GONG Wen-ping;WANG Can-guo;CHENG Dun-gong;LIU Ai-feng;CAO Xin-you;LIU Cheng;ZHAI Sheng-nan;YANG Zu-jun;LIU Jian-jun;KONG Ling-rang;ZHAO Zhen-dong;SONG Jian-min(Crop Research Institute,Shandong Academy of Agricultural Sciences(SAAS)/National Engineering Laboratory for Wheat and Maize/Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow and Huai River Valley,Ministry of Agriculture,Jinan 250100;College of Agronomy,Ludong University,Shandong Yantai 264025;Shandong Seed Administration Station,Jinan 250100;College of Agronomy,Shandong Agricultural University/State Key Lab of Crop Biology,Taian 271018;School of Life Science and Technology,University of Electronic Science and Technology,of China,Chengdu 610054)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2019年第4期854-860,共7页
Journal of Plant Genetic Resources
基金
山东省自然科学基金博士基金项目(ZR2018BC031)
山东省农业科学院创新工程(CXGC2018E01)
国家产业技术体系(CARS-03-07)
中国科协青年人才托举工程(2017-2019)
泰山学者种业计划(2014-2019)
作物生物学国家重点实验室开放课题(2017KF03)~~
关键词
小麦
二倍体长穗偃麦草
1E^e染色体
农艺和品质性状
分子标记
common wheat
Thinopyrum elongatum(Host)D.R.Dewey
chromosome 1E^e
agronomical and quality-related traits
molecular markers