百里香酚对脂多糖诱导的子宫内膜上皮细胞炎性反应的抑制作用研究

Inhibiting Effect of Thymol on Inflammation Induced by LPS in Endometrial Epithelial Cells

为探究百里香酚治疗子宫内膜炎的抗炎活性,利用脂多糖(LPS)诱导山羊子宫内膜上皮细胞(gEECs)建立炎性模型,以百里香酚进行干预。采用NO试剂盒和酶联免疫吸附试验(ELISA)分别检测百里香酚对LPS诱导的NO和细胞因子分泌的影响;利用实时荧光定量PCR(RT-PCR)检测百里香酚对LPS诱导的IL-1β、TLR4和NF-κB基因转录的影响。结果显示:不同剂量的百里香酚(100、50、25μg·mL^-1)能不同程度地抑制LPS诱导的炎性模型细胞TNF-α、IL-1β、IL-6、NO和PGE2的分泌,亦可降低炎性模型细胞IL-1β、TLR4和NF-κB的基因转录水平(P<0.01)。结果表明,百里香酚可抑制LPS诱导gEECs炎性模型的细胞因子表达水平,具有显著的抗炎活性,其作用机制可能与抑制TLR4/NF-κB信号通路相关。

In order to investigate the anti-inflammatory effect of thymol on endometritis, the inflammatory model was established in LPS-induced goat endometrial epithelial cells(gEECs)with thymol treantment. The secretion of NO and cytokines were measured by NO kit and ELISA kits respectively in LPS-induced gEECs. Gene transcription of IL-1β, TLR4, and NF-κB were detected by RT-PCR respectively in LPS-induced gEECs. The results showed that thymol(100, 50, 25 μg·mL^-1) inhibited the secretion of TNF-α, IL-1β, IL-6, NO, and PGE2 to a significant extent in LPS-induced gEECs(P<0.01). In addition, thymol markedly decreased gene transcription of IL-1β, TLR4, and NF-κB in LPS-induced gEECs(P<0.01). These results demonstrated that thymol exerted anti-inflammatory effect through inhibiting the level of inflammatory cytokines, which possibly involved in the inhibition of TLR4/NF-κB signaling pathway.