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家兔小肠不同区段营养物质转运载体相关基因的分布规律 被引量:4

Distribution of Nutrient Transporter Related Genes in Different Segments of Small Intestine of Rabbits
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摘要 旨在研究家兔小肠不同区段营养物质转运载体相关基因的分布规律。本研究选取110日龄体重相近的健康白色獭兔10只,屠宰后采集十二指肠、空肠、回肠样品,采用real-time PCR研究家兔不同肠段小肽转运载体Pep T1,氨基酸转运载体CAT1、B^0AT、EAAT3、rBAT,葡萄糖转运载体SGLT1、GLUT2、GLUT5,以及脂肪酸转运载体FATP4的mRNA表达丰度。结果显示,小肽转运载体Pep T1 mRNA在十二指肠表达量最高,空肠略低;碱性氨基酸转运载体CAT1、兼性氨基酸转运载体rBAT和中性氨基酸转运载体B^0AT mRNA的表达量均在回肠最高,空肠次之;酸性氨基酸转运载体EAAT3 mRNA的表达量在空肠和回肠均较高;葡萄糖转运载体SGLT1和GLUT5 mRNA的表达量在十二指肠和空肠均较高;葡萄糖转运载体GLUT2和脂肪酸转运载体FATP4 mRNA的表达量则是空肠最高,十二指肠次之。结果表明,家兔肠道转运吸收小肽、葡萄糖和脂肪酸的主要部位是小肠前半段,转运吸收氨基酸的主要部位是小肠后半段。 The objective of this study was to investigate the distribution of nutrient transporter related genes in different segments of small intestine of rabbit. Ten healthy white rex rabbits at 110 days old with similar weight were chose to slaughter. The intestinal segment samples were collected from duodenum, jejunum, ileum for detecting the mRNA abundance of oligopeptide transporter Pep Tl, amino acid transporter CAT1, B^0AT, EAAT3, rBAT, glucose transporter SGLT1, GLUT2, GLUT5 and fatty acid transporter FATP4 by real-time PCR. The results showed that the abundance of oligopeptide transporter Pep T1 mRNA was the highest in duodenum, and lower in jejunum. The abundance of basic amino acid transporter CAT1, facultative amino acid transporter rBAT, and neutral amino acid transporter B^0AT mRNA were the highest in ileum, and lower in jejunum. The acidic amino acid transporter EAAT3 mRNA expression was higher in jejunum and ileum. The expression of glucose transporter SGLT1 and GLUT5 mRNA was higher in duodenum and jejunum. The abundance of glucose transporter GLUT2 and fatty acid transporter FATP4 mRNA were the highest in jejunum, and lower in duodenum. The result indicate that the main part in intestine for transporting and absorbing oligopeptide, glucose and fatty acid is the first half of the small intestine, the main part for transporting and absorbing amino acid is the posterior segment of the small intestine.
作者 陈赛娟 刘亚娟 袁万哲 李江涛 庞立欣 谷子林 陈宝江 CHEN Saijuan;LIU Yajuan;YUAN Wanzhe;LI Jiangtao;PANG Lixin;GU Zilin;CHEN Baojiang(Mountainous Area Research Institute of Hebei Province,Hebei Agricultural University,Baoding 071001,China;Hebei Agricultural Engineering Technology Research Center inMountainous Area,Baoding 071001,China;College of Veterinary Medicine,HebeiAgricultural University,Baoding 071001,China;College of Animal Science and Technology,Hebei Agricultural University,Baoding 071001,China)
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2019年第7期1518-1524,共7页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 河北省高等学校科学技术研究项目(bj2014035) 河北省青年拔尖人才支持计划(2016) 国家兔产业技术体系建设项目(CARS-43-B-2)
关键词 家兔 小肠 营养物质转运载体 mRNA表达丰度 rabbit small intestine nutrient transporter mRNA expression abundance
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