海洋鱼骨胶原低聚肽与天门冬氨酸钙联用增加去卵巢大鼠骨密度

Marine fish bone collagen oligopeptide combined with calcium aspartate increases bone mineral density in obariectomized rats

目的探讨海洋鱼骨胶原低聚肽与天门冬氨酸钙单独及联合应用对去卵巢大鼠骨密度的改善效果。方法 3月龄Wistar雌性大鼠60只,按体重随机分为6组:假手术组、去卵巢模型对照组、天门冬氨酸钙组、海洋鱼骨胶原低聚肽组、天门冬氨酸钙+海洋鱼骨胶原低聚肽组、碳酸钙阳性对照组,每组10只。假手术组和去卵巢模型对照组每天灌胃给予相同体积纯水,其余各组灌胃给予相应受试物:天门冬氨酸钙116.7 mg/kg、海洋鱼骨胶原低聚肽250 mg/kg、天门冬氨酸钙116.7 mg/kg+海洋鱼骨胶原低聚肽250 mg/kg、碳酸钙35.6 mg/kg,连续灌胃90 d后处死动物,取肝脏和肾脏计算脏器系数并进行病理学检查,取股骨测定骨密度和骨钙含量,取血清测定骨代谢生化指标血清钙、磷浓度和碱性磷酸酶(ALP)活性。结果模型组骨密度和骨钙含量均显著低于假手术组(P<0.05),表明采用卵巢切除术成功建立骨质疏松模型。各组大鼠脏器指数差异均无统计学意义(P>0.05),肝脏/肾脏组织学镜检均未见异常改变。天门冬氨酸钙+海洋鱼骨胶原低聚肽组的骨密度显著大于模型组(P<0.05),天门冬氨酸钙组、海洋鱼骨胶原低聚肽组的骨密度大于去卵巢模型组,但差异无统计学意义(P>0.05);与去卵巢模型组相比,天门冬氨酸钙+海洋鱼骨胶原低聚肽组的骨钙含量显著增高(P<0.05)。与天门冬氨酸钙组相比,天门冬氨酸钙+海洋鱼骨胶原低聚肽组的骨钙含量较高(P<0.05);各组血清钙浓度差异无统计学意义(P>0.05),与去卵巢模型组相比,天门冬氨酸钙组、海洋鱼骨胶原低聚肽组、天门冬氨酸钙+海洋鱼骨胶原低聚肽组大鼠血清磷浓度显著升高(P<0.05),ALP活力显著降低(P<0.05)。结论天门冬氨酸钙与海洋鱼骨胶原低聚肽联合使用有明显的增加骨密度作用,表明海洋鱼骨胶原低聚肽对天门冬氨酸钙的吸收有一定促进作用。

OBJECTIVE To investigate the effect of marine fish collagen oligopeptide and calcium aspartate alone or in combination on bone mineral density in ovariectomized rats. METHODS Sixty three-month-old SPF Wistar female rats were randomly divided into 6 groups according to their body weight: sham operation group, model control group(ovariectomy), calcium aspartate group(ovariectomy), marine fish collagen oligopeptide group(ovariectomy), aspartate calcium + marine fish collagen oligopeptide group(ovariectomy) and calcium carbonate control group(ovariectomy), 10 rats in each group. The sham operation group and the model control group were given the same volume of pure water by gavage, and the other groups were intragastrically administered with calcium aspartate(116.7 mg/kg), marine fish collagen oligopeptide(250 mg/kg), calcium aspartate(116.7 mg/kg)+ marine fish collagen oligopeptide(250 mg/kg), calcium carbonate(35.6 mg/kg), and the test substance was continuously administered for 90 days. After 90 days, the animals were sacrificed, and the liver and kidney of the rats were taken to calculate the organ coefficient and pathological examination. The rat femurs were taken to measure bone mineral density and bone calcium content and rat serum was used to determine serum calcium, phosphorus concentration and alkaline phosphatase(ALP) activity. RESULTS Bone mineral density and bone calcium content in the model group were significantly lower than those in the sham operation group(P<0.05), indicating that the osteoporosis model was successfully established by oophorectomy. There was no significant difference in the organ index of each group(P>0.05), liver/kidney HE staining microscopic examination showed no abnormal changes, indicating the safety of the test substances. The bone mineral density of the aspartate calcium + marine fish collagen oligopeptide group was significantly greater than that of the model group(P<0.05).The bone mineral density of the aspartate calcium group and the marine fish collagen oligopeptide group was larger than that of the model group, but there was no significant difference(P>0.05). Compared with that in the model group, the calcium content of calcium aspartate + marine fish collagen oligopeptide group was significantly higher(P<0.05). Compared with that in the calcium aspartate group, the calcium content of calcium aspartate + marine fish collagen oligopeptide group was higher(P<0.05), there was no significant difference in serum calcium concentration between groups(P>0.05).Compared with that in the model group, serum phosphorus concentration in the aspartate calcium group, marine fish collagen oligopeptide group, aspartate calcium + marine fish collagen oligopeptide group was significantly higher(P<0.05) and ALP activity was significantly reduced(P<0.05). CONCLUSION The combination of calcium aspartate and marine fish collagen oligopeptide has a significant effect on increasing bone mineral density, also indicating that marine fish bone collagen oligopeptide could promote absorption of calcium aspartate.