血液中脂肪细胞膜特异性蛋白ASC-1的来源探讨

Discussion on the source of adipocyte membrane protein ASC-1 in blood circulation

目的提出从分子水平对脂肪栓塞进行诊断的新思路,初步探索脂肪细胞膜特异性蛋白ASC-1对脂肪栓塞的诊断价值。方法纳入来自北京积水潭医院的30例受试者。取10例非手术患者和关节置换术前患者的血清;取10例行关节置换术患者术后24h的血清;取5例行关节置换术患者的皮下脂肪,5例行关节置换术患者关节腔内脂肪;取10例行关节置换术患者的黄骨髓。采用酶联免疫吸附实验检测以上标本中 ASC-1水平,并进行描述性分析。结果非手术的患者血清中均未检测到ASC-1,2例关节置换术前患者血清检测到 ASC-1,3例关节置换术后患者血清中检测到ASC-1,5例皮下脂肪均检测到 ASC-1,1例关节腔内脂肪组织检测到ASC-1,5例黄骨髓检测到ASC-1。结论脂肪细胞膜特异性蛋白ASC-1可能成为FE诊断的特异性指标,但需进一步研究以证实其临床应用价值。

Objective A new idea of molecular level diagnosis of fat embolism was proposed. To study the diagnostic value of adipocyte protein A SC-1 in the diagnosis of fat embolism. Methods Thirty subjects from Beijing Jishuitan Hospital were enrolled. The ASC-1 level in the specimens was detected by ELISA:serum of 10 non-surgical patients and before orthopaedic surgery patients, serum of 10 patients after joint replacement, subcutaneous adipose tissue of 5 patients, adipose tissue in the articular cavity from 5 patients, and yellow bone marrow of 10 patients. Descriptive analysis was conducted. Results No ASC-1 was found in the serum of non-operative patients. ASC-1 was found in serum of 2 patients before orthopaedic surgery. ASC-1 was found in 3 patients after orthopaedic surgery. ASC-1 was found in subcutaneous adipose tissue of the lower extremity. ASC-1 was found in the intraluminal adipose tissue of 1 case. ASC-1 was found in 5 cases of yellow bone marrow. Conclusions Adipocyte membrane specific protein ASC-1 may be a specific indicator of FE diagnosis. Howere, further studies are needed to confirm its clinical application value.