原发性胆囊癌患者血清miR-335和miR-155水平及其临床意义

Serum levels and significances of miR-335 and miR-155 in primary gallbladder cancer

目的探讨原发性胆囊癌(PCG)患者血清微小RNA-335(miR-335)和微小RNA-155(miR-155)水平及其临床意义.方法选取2016年1月至2018年10月海南省第二人民医院收治的96例PCG患者(PCG组)和50例体检健康者(对照组),采用实时定量PCR(RT-PCR)检测各组血清miR-335和miR-155水平,分析血清miR-335和miR-155水平与PCG患者临床病理特征的关系.应用ROC曲线分析miR-335和miR-155对PCG的诊断价值.结果 PCG组血清miR-335水平明显低于对照组(1.50 ±0.42∶3.65±1.18,t=10.319,P<0.001),PCG组血清miR-155水平明显高于对照组(3.18±0.61∶0.74±0.12,t=13.627,P<0.001).在PCG患者中血清miR-335和miR-155水平均与TNM分期(t=4.863,P=0.024;=5.117,P=0.008)及淋巴结转移(t=5.725,P<0.001;t=6.802,P<0.001)相关.ROC曲线分析显示,miR-335和miR-155诊断PCG的临界值分别为1.18、2.35,两项联合诊断PCG的曲线下面积最大,为0.920,95% CI为0.863 ~0.977,其敏感性和特异性为93.8%和85.7%.结论血清低水平miR-335和高水平miR-155与PCG较高的TNM分期及淋巴结转移相关,两项联合检测有助于提高PCG诊断率.

Objective To investigate the serum levels and clinical significances of microRNA-335 (miR-335) and microRNA-155 (miR-155) in patients with primary gallbladder cancer (PCG).Methods A total of 96 PCG patients (PCG group) and 50 healthy controls (control group) admitted to the Second People's Hospital of Hainan Province from January 2016 to October 2018 were selected.Real-time quantitative PCR (RT-PCR) was used to detect the serum levels of miR-335 and miR-155 in each group.The relationships between miR-335 and miR-155 levels and clinical pathological characteristics of PCG patients were analyzed.The diagnostic value of miR-335 and miR-155 in PCG was analyzed by ROC curve.Results The serum level of miR-335 in PCG group was significantly lower than that in the control group (1.50 ± 0.42 vs.3.65 ± 1.18,t =10.319,P <0.001).The serum level of miR-155 in PCG group was significantly higher than that in the control group (3.18 ±0.61 vs.0.74±0.12,t =13.627,P<0.001).The serum levels ofmiR-335 and miR-155 in PCG patients were correlated with TNM stage (t =4.863,P =0.024;t =5.117,P =0.008) and lymph node metastasis (t =5.725,P < 0.001;t =6.802,P < 0.001).ROC curve analysis showed that the critical values of serum miR-335 and miR-155 for diagnosing PCG were 1.18 and 2.35,respectively.The area under the curve of the two combined diagnosis of PCG (0.920,95% CI:0.863-0.977) was the largest,with sensitivity and specificity of 93.8% and 85.7%.Conclusion The low serum level of miR-335 and high level of miR-155 are associated with the higher TNM stage and lymph node metastasis of PCG,and the combined detection of the two is helpful to improve the diagnostic rate of PCG.