实时荧光定性聚合酶链式反应标准方法检测转基因产品的验证及应用

Validation and application of qualitative real-time polymerase chain reaction methods for the detection of genetically modified organisms and derived products

目的验证实验室大豆、玉米和水稻及其制品转基因检测方法,并应用于实际样品检测。方法根据GB 19495.4-2018《转基因产品检测实时荧光定性聚合酶链式反应(PCR)检测方法》要求对无转基因标识的样品进行转基因成分检测。结果方法验证满意。40批次样品(大豆、玉米和水稻及其制品)中发现1批次的转基因成分检出,检出率为2.5%。结论市场中绝大部分未标示转基因成分的大豆、玉米和水稻及其制品确实未检出转基因成分,仅有极少数产品含有转基因成分,但未进行有效标识。

Objective To verify the genetically modified(GM) plant detection methods for soybean, maize, rice and their products, and applied to actual sample testing.Methods The genetically modified ingredients of the no GM labeling products was detected according to GB 19495.4-2018 Detection of genetically modified organisms and derived products-Qualitative real-time polymerase chain reaction(PCR) method.Results This method validation was satisfactory.One batch of GM component was detected in 40 batches of soybean, corn, rice and their products,and the detection rate was 2.5%.Conclusion The vast majority of soybeans, corn, rice and their products in the market those are not labeled with GM ingredients do not show GM ingredients.Only a few of them contain genetically modified ingredients, but they are not effectively labeled.