姜黄中姜黄素类化合物提取工艺研究

Study on extraction process of curcuminoids from turmeric

目的研究不同提取条件对姜黄中姜黄素类化合物提取率的影响,优化提取工艺。方法采用单因素实验研究姜黄粒径、液料比、乙醇浓度、超声时间和超声功率对姜黄素类化合物提取率的影响,并利用响应面分析优化总姜黄素的提取工艺。结果单因素实验结果表明粒径、液料比、乙醇浓度对姜黄素类化合物的提取率影响显著(P<0.05),而超声时间和超声功率对提取率的影响不显著(P>0.05)。利用响应面分析拟合总姜黄素提取率的回归方程,得到最佳提取工艺为:粒径0.18 mm,液料比76.05(mL/g),乙醇浓度68.15%,在此条件下得到最佳提取率为1.062%,验证试验得到提取率实测值为1.017%,与预测值基本吻合,说明响应面模型与优化条件准确可靠。结论本研究为姜黄中姜黄素类化合物的提取工艺的优化提供了理论依据。

Objective To study the effect of different extraction conditions on the extraction rate of curcumin compounds in turmeric and optimize the extraction process.Methods The effects of turmeric particle size,liquid-to-liquid ratio, ethanol concentration, ultrasonic time and ultrasonic power on the extraction rate of curcuminoids were studied by single factor experiment, and the optimum extracting condition was obtained utilizing response surface method.Results The results of single factor tests showed that particle size, liquid-to-solid ratio and ethanol concentration had significant effects on extraction rate(P<0.05), while the ultrasonic time and power had no effects on it(P>0.05).A regression equation with extraction rate as response was obtained using response surface optimization, and the optimum extracting conditions were as follows: particle size was 0.18 mm, liquid-to-solid ratio was 76.05(mL/g), ethanol concentration was 68.15%, and the optimum extraction rate was 1.062% correspondingly.The verification tests showed that the extraction rate was 1.017%, which was accordant with predicted value, and it indicated that the response surface model and optimizing conditions were reliable.Conclusion This study provides theoretical basis for the extraction process optimization of curcuminoids from turmeric.