无花果中维生素C含量的测定

Determination of vitamin C content in Ficus carica L.

目的采用分光光度法测定无花果和无花果干中维生素C的含量。方法选取一定浓度维生素C标准液进行波长扫描,确定维生素C的最大吸收波长,将待测样品匀浆处理后在此波长下进行分光光度测定。结果维生素C的最大吸收波长为520 nm,校准曲线方程Y=0.0208X+0.001(r^2=0.9998),无花果和无花干果中维生素C含量分别为69.08 mg/100 g和64.06 mg/100 g,加标回收实验中回收率均在90%以上。结论无花果中含有较高的维生素C,且无花果维生素C含量高于无花果干中维生素C含量。

Objective To determine the content of vitamin C in Ficus carica L.and dry figs.Methods The certain concentration vitamin C standard solution was used for wavelength scanning to determine the maximum absorption wavelength of vitamin C.The homogenized sample was determined by spectrophotometry at this wavelength.Results The maximum absorption wavelength of vitamin C was 520 nm, the calibration curve equation was Y=0.0208X+0.001(r^2=0.9998), and the vitamin C content in Ficus carica and dried Ficus carica was 69.08 mg/100 g and 64.06 mg/100 g.The recoveries were above 90%.Conclusion Ficus carica L.has higher vitamin C content, and the vitamin C content in Ficus carica is higher than dry figs.