转录因子叉头框C2基因C-512T多态性与非酒精性脂肪性肝病患者相关代谢因素的分析

Analysis between FOXC2 gene C-512T polymorphism and metabolic factors in patients with non-alcoholic fatty liver disease

目的探讨转录因子叉头框C2(FOXC2)基因C-512T多态性与非酒精性脂肪性肝病(NAFLD)及其相关代谢因素的关系。方法应用聚合酶连反应-限制性片段长度多态性(PCRRFLP)方法,对269名正常对照组(NC)和237例NAFLD患者(NAFLD组)的FOXC2基因C-512T多态性进行测定,同时进行体表测量、生化指标及腹部B超检查。结果与NC组比较,NAFLD组FOXC2基因C-512T多态性的基因型频率分布差异无统计学意义(χ^2=4.455,P=0.108);而NAFLD组T等位基因频率降低(χ^2=3.638,P=0.048);NAFLD组CC型患者TG、HOMA-IR高于TT型患者,差异有统计学意义(P<0.05)。Logistic回归分析显示,CC基因型和HOMA-IR是NAFLD的影响因素。结论FOXC2基因C-512T多态性CC基因型可能与NAFLD发生有关。

Objective To investigate the relationship between FOXC2 gene C-512 T polymorphism and metabolic factors in patients with non-alcoholic fatty liver disease(NAFLD).Methods A total of506 subjects were enrolled in this study and divided into two groups:normal control(NC)group(n=269)and NAFLD group(n=237). FOXC2 gene C-512 T polymorphism was tested by restriction fragment length polymorphism(PCR-RFLP)in all the subjects. Meanwhile,physical examination,biochemical tests and abdominal ultrasound examination were tested.Results There was no significant difference between genotypes frequency distribution of the FOXC2 gene C-512 T polymorphism in the NAFLD group and NC group(χ^2=4. 455,P=0. 108). The T allele frequency was lower in the group of NAFLD than in NC group(χ^2= 3. 638,P=0. 048). TG and HOMA-IR were higher in CC than in TT genotype in NAFLD group(P<0. 05). Logistic analysis showed that CC genotype and HOMA-IR were the risk factors for NAFLD.Conclusion CC genotype of FOXC2 gene C-512 T polymorphism may be involved in the pathogenesis of NAFLD.