电针联合运动对肥胖大鼠骨骼肌过氧化物酶体增殖物激活受体γ辅激活因子1α、鸢尾素、腺苷酸活化蛋白激酶的影响

Effect of electroacupuncture combined with treadmill exercise on body weight and expression of PGC-1α,Irisin and AMPK in skeletal muscle of diet-induced obesity rats

目的:观察电针联合运动疗法对高脂饮食诱导的肥胖(DIO)大鼠骨骼肌过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)、鸢尾素(Irisin)、腺苷酸活化蛋白激酶(AMPK)表达的影响,探讨电针联合运动改善肥胖的机制。方法:SD大鼠随机分为对照组(10只)和高脂组(55只)。采用高脂饮食喂养复制肥胖大鼠模型。高脂组中成功造模的肥胖大鼠随机分为模型组、运动组、电针组和电针联合运动组,每组8只。电针联合运动组和电针组电针"足三里""天枢"穴,每次30min,每周5次,共8周。电针联合运动组和运动组采用跑台训练,16m/min,每天60min,每周5次,共8周。每周测量各组大鼠体质量,采用荧光定量PCR法检测大鼠腓肠肌中PGC-1α、Ⅲ型纤连蛋白组件包含蛋白5(FNDC5)、AMPK mRNA的表达,Western blot法检测大鼠腓肠肌中PGC-1α、FNDC5的表达水平及AMPK的磷酸化水平。结果:模型组大鼠体质量明显高于对照组(P<0.05);PGC-1α、FNDC5mRNA及蛋白表达水平,AMPK mRNA及磷酸化水平明显低于正常组(P<0.05)。电针组、运动组、电针联合运动组体质量明显低于模型组(P<0.05),PGC-1α、FNDC5mRNA及蛋白表达水平,AMPK mRNA及磷酸化水平明显高于模型组(P<0.05)。电针联合运动组体质量明显低于电针组及运动组(P<0.05),PGC-1α、FNDC5mRNA及蛋白表达水平,AMPK mRNA及磷酸化水平明显高于电针组及运动组(P<0.05)。结论:电针和运动均可促进DIO大鼠骨骼肌中PGC-1α、Irisin的表达及AMPK磷酸化水平,且电针联合运动疗效更好,提示电针联合运动疗法可能是通过恢复骨骼肌细胞脂肪酸氧化、改善线粒体功能从而实现减肥的。

Objective To investigate the effect of electroacupuncture(EA)plus treadmill exercise on the expression of peroxisome proliferator activated receptorγcoactivator 1α(PGC-1α),Irisin,AMP-activated protein kinase(AMPK)in skeletal muscle of diet-induced obesity(DIO)rats,so as to explore its mechanism underlying body reduction promotion.Methods Forty-two male SD rats were divided into normal diet(control,n=10),high fat diet(model),EA,treadmill exercise and EA plus treadmill exercise(combination)groups(n=8 in each of the latter 4 groups).The obesity model was established by feeding the rats with high fat diet.EA(2 Hz/15 Hz,1 mA)was applied to bilateral"Zusanli"(ST36)and"Tianshu"(ST25)for 30 min,5 times per week for a total of 8 weeks.Rats of the treadmill exercise group were forced to perform exercise on a treadmill(16 m/min)for 30 min,5 times per week for a total of 8 weeks.Rats in the combination group received the above-mentioned two methods.During the treatment,rats in the control group were fed with normal fodder,rats in other groups were fed with high fat fodder,and their body weight was measured once a week.The expression levels of PGC-1α,fibronectin typeⅢ domain containing 5(FNDC5),AMPK mRNA and protein of skeletal muscle were measured by quantitative real-time PCR and Western blot,respectively.Results After modeling,the body weight was significantly increased(P<0.05),and the expression levels of PGC-1αand FNDC5 mRNA and protein,AMPK mRNA and phosphorylated AMPK(p-AMPK)protein in the skeletal muscle were considerably decreased in the model group relevant to the control group(P<0.05).Following the treatment,the body weight was significantly down-regulated,while the expression levels of PGC-1αand FNDC5 mRNAs and proteins,AMPK mRNA and p-AMPK protein were obviously upregulated in the EA,treadmill exercise and combination groups relevant to the model group(P<0.05).The therapeutic effect of EA plus treadmill exercise was significantly superior to those of both simple EA and simple treadmill exercise in down-regulating the body weight,as well as in up-regulating the expression of PGC-1αand FNDC5 mRNAs and proteins,AMPK mRNA,and p-AMPK protein(P<0.05).Conclusion Both EA and treadmill exercise can significantly increase the expression of PGC-1α,FNDC5 and p-AMPK in skeletal muscle of DIO rats,suggesting their efficacy in restoring fatty acid oxidation in skeletal muscle cells and improving mitochondrial function,which may contribute to their function in body reduction.The therapeutic effect of EA plus treadmill exercise is better than that of simple EA and simple treadmill exercise.