决明子水煎提取工艺优化及其颗粒剂中指标成分量值转化研究

Optimization of Water Extraction Process of Cassiae Semen and the Quantity Transfer of Index Components in Cassiae Semen Formula Granules

目的优化决明子配方颗粒的制备工艺及进行指标成分量值转化研究。方法采用L9(3^4)正交试验,以出膏率、橙黄决明素及大黄酚含量为综合评价指标,以浸泡时间、加水量、提取时间及提取次数为考察因素,优选水煎提取工艺。饮片经最佳工艺提取后,经减压浓缩、喷雾干燥、干法制粒等制成配方颗粒。通过指标成分的含量测定比较饮片-提取液-干粉-配方颗粒间指标成分量值转化的相关性。结果最佳水煎提取工艺为浸泡0.5h,加10倍量水,煎3次,每次1.5h;橙黄决明素及大黄酚总转移率分别为76.59%~79.31%,12.85%~15.97%。结论决明子提取工艺稳定,重复性好。提取液、干粉及配方颗粒中橙黄决明素和大黄酚的量值转化过程一致性较好,可为大规模生产提供依据。

Objective To optimize the preparation process of Cassiae Semen Formula Granules and to study its quantity transfer of index components.Methods Taking the paste rate,the contents of aurantio-obtusin and chrysophanol as comprehensive evaluation indexes and taking soaking time,water addition,extraction time and extraction times as factors,L9(3^4) orthogonal test was used to select the optimum water extraction process.After extracting the decoction pieces by the optimum process,the formula granules were prepared by vacuum concentration,spray drying and dry granulation.The correlation between the amount transfer of index components among the decoction piece-extract solution-dry powder-formula granules was compared by the content determination of index components.Results The optimum extraction process was as follows: soaking for 0.5 h,adding 10 times the amount of water,decocting for 3 times,1.5 h each time.The total transfer rate of aurantio-obtusin was in the range of 76.59%-79.31% and that of chrysophanol was in the range of 12.85%-15.97%.Conclusion The extraction process of Cassiae Semen is stable and reproducible.The content of aurantio-obtusin and chrysophanol in extract,dry powder and formula granules showed a quantitative transfer relationship,which has a good consistency and can provide a basis for large-scale production.