甘蓝型油菜种子萌发期耐铝毒特性综合评价及其种质筛选

Screening and comprehensive evaluation of aluminum-toxicity tolerance during seed germination in Brassca napus

种子萌发是油菜植株形态建成的重要阶段,铝毒是酸性土壤中影响其种子萌发的主要因素之一,因此筛选出种子萌发过程中耐铝毒种质对油菜生产及研究具有重要意义。本研究利用5份甘蓝型油菜品种(系)筛选出油菜萌发期耐铝毒种质资源处理的适宜胁迫浓度为90μg mL^-1。并以该浓度处理148份甘蓝型油菜品种(系)种子,于萌发期测定其发芽势、发芽率、鲜重、干重、根长和芽长等指标,通过铝毒胁迫耐性综合评价值(A 值)、平均隶属函数值(ASF值)、对铝毒加权耐性系数(WAC 值)、相关性、频数、主成分、灰色关联度、聚类和逐步回归分析等鉴定萌发期耐铝性,建立萌发期对铝毒耐性综合评价模型并筛选出适宜的评价鉴定指标。结果表明,148份甘蓝型油菜的萌发期各指标在品种(系)间存在显著差异;筛选出萌发期耐铝毒甘蓝型油菜品种(系) 01188、WH-20、A109、甲预31 棚等。根据灰色关联度及回归分析结果认为,在油菜萌发期测定其根长、芽长、鲜重、发芽率和发芽势,通过回归方程估算其A值,可以初步判断甘蓝型油菜种质的耐铝毒特性。

Seed germination is an important stage of plant morphogenesis in rapeseed.Aluminum toxicity is one of the main factors affecting seed germination in acidic soil.Therefore,it is important for rapeseed production and research to screen Al-tolerant germplasms during seed germination.In this study,five Brassica napus cultivars (lines) were used to screen the optimum treatment concentration of Al toxicity at germination stage,which was determined at 90 μg mL^-1.The germination vigor,germination rate,fresh weight,dry weight,root length and bud length of 148 cultivars (lines) of Brassica napus were measured at germination stage.The comprehensive evaluation value (A value),average subordinate function value (ASF value),weighted tolerance coefficient (WAC value),correlation coefficient,frequency,principal component,grey relational degree,clustering and stepwise regression were used to identify the Al tolerance at germination stage.A comprehensive evaluation model of Al tolerance at germination stage was established,suitable evaluation and identification indexes were selected.There were significant differences in the germination parameters of 148 Brassica napus germplasm,and aluminum-tolerant Brassica napus varieties (lines) during germination stage were screened,including 01188,WH-20,A109,and Jiayu 31 peng.The results of grey correlation and regression analysis indicated that the root length,bud length,fresh weight,germination rate and germination potential measured at the germination stage of rapeseed,and the A value of the indices estimated by regression equation,could be preliminarily judged the aluminum tolerance of rape germplasm.