miR-155在胶质瘤干细胞样细胞的表达及临床意义

The expression of miR-155 in GSCs and its clinical significance

目的 研究miR-155在人胶质瘤干细胞样细胞(glioblastoma stem-like cells,GSCs)与正常神经干细胞(neural stem cells,NSCs),及不同病理级别脑胶质瘤组织的表达差异,其表达水平与胶质瘤恶性程度及预后的关系。方法 采用神经球(neurosphere)培养法体外分离扩增人GSCs与NSCs,并进行免疫荧光及移植瘤实验鉴定。用qRT-PCR的方法检测miR-155在GSCs、 NSCs及不同级别胶质瘤和正常脑组织的表达水平。结合患者的预后,进行Kaplan-Meier生存曲线分析。结果 GSCs的miR-155表达水平明显高于NSCs (P<0.05);随着胶质瘤级别的增高,miR-155的表达水平也明显增高,WHO Ⅲ级、Ⅳ级恶性胶质瘤的miR-155表达水平明显高于正常脑组织(均P<0.05)。miR-155高表达与恶性胶质瘤的不良预后密切相关。结论 miR-155在GSCs及高级别胶质瘤组织中的表达水平增高,其表达水平可作为判定胶质瘤预后的标志物。

Objective To study the different expression of miR-155 in glioblastoma stem-like cells(GSCs),neural stem cells(NSCs) and different grades glioma.To clarify the relationship of miR-155 expression,degree of malignancy and prognosis of glioma patients.Methods GSCs and NSCs were isolated in vitro using neurosphere culture,then characteristics of the GSCs and NSCs were identified by experiments of immunoflurescence and xenograft.QRT PCR technology was used to detect the expression levels of miR-155 in GSCs,NSCs,gliomas of different grades and normal brain tissues.Kaplan-meier survival curve analysis was performed in combination with patient prognosis.Results The expression of miR-155 increased significantly in GSCs compared with NSCs(P<0.05),and also increased with the malignant degree of glioma.The expression level of miR-155 in malignant glioma(WHO Ⅲ,Ⅳ) was obviously higher than that of normal brain tissue (allP<0.05).The high expression of miR-155 was closely related to the poor prognosis of glioma.Conclusion The expression of miR-155 elevates in GSCs and malignant glioma specimens,and can be considered as biomarker for diagnosis and prognosis judgment of glioma.