摘要
以Fe3O4为核,采用层层自组装的方法合成核壳型Fe3O4@MIL-100(Fe)磁性纳米材料,经高温煅烧得到具有较大孔径(17.78 nm)、高含碳量(6.79%)的磁性纳米材料Fe3O4@MC。将该材料用于多肽的富集,并通过基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF MS)进行鉴定。Fe3O4@MC材料从牛血清白蛋白(BSA)酶解液中富集得到33条肽段,且具有较高的选择性(BSA酶解液与BSA的质量比为1∶400),用于人血清中内源性肽段的分离富集也取得了较好的效果。
Core-shell magnetic nanoparticles,Fe 3O 4@MIL-100(Fe),were synthesized by layer -by-layer self-assembly.The final material,Fe 3O 4@MC,was obtained after high temperature calcination.Fe 3O 4@MC magnetic nanoparticles exhibited large pore size (17.78 nm) and high carbon content (6.79%).Thirty-three peptides of bovine serum albumin (BSA) digest were enriched by Fe 3O 4@MC magnetic nanomaterial.Fe 3O 4@MC magnetic nanomaterial also showed high selectivity for peptides when the mass ratio of BSA digest to BSA was 1∶400.In addition,the nanomaterial material exhibited excellent enrichment performance for the endogenous peptides of human serum.
作者
赵雅梦
王敏
张凌怡
鲁誉浩
张维冰
ZHAO Yameng;WANG Min;ZHANG Lingyi;LU Yuhao;ZHANG Weibing(School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China)
出处
《色谱》
CAS
CSCD
北大核心
2019年第8期831-835,共5页
Chinese Journal of Chromatography
基金
国家自然科学基金(21475044,51472121)~~
关键词
基质辅助激光解吸电离飞行时间质谱
富集
金属有机框架
内源性肽段
matrix-assisted laser desorption ionization-time of flight mass spectrometry ( MALDI -TOF MS)
enrichment
metal-organic framework (MOFs)
endogenous peptides
