苗药血人参提取物对衰老小鼠学习能力的影响

The Effect of Indigofera Stachyoides Extract from Miao Medicine on Learning Ability of Aging Mice

目的:探讨血人参提取物对衰老小鼠学习能力的影响及其可能的作用机制。方法:制备衰老小鼠模型,将其分为正常对照组、模型组、阳性对照组(脑复康0.8g·kg^-1·d^-1)、血人参高、中、低剂量组(20g·kg^-1·d^-1、10g·kg^-1·d^-1、5g·kg^-1·d^-1),观察血人参提取物对衰老小鼠体重变化的影响和学习能力的影响。采用ELISA法检测衰老小鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)的含量。结果:与模型组比较,从第2周开始,血人参高、中、低剂量组体重出现明显增加(P<0.05);中、低剂量组小鼠学习能力显著提高(P<0.05);与模型组比较,血人参高、中、低剂量组血清中GSH-Px含量显著升高(P<0.01)。结论:血人参提取物抗衰老效果明显,能显著提高衰老小鼠的抗氧化能力和学习能力。

Objective:To investigate the effect of Indigofera stachyoides extract on learning ability of aging mice and its possible mechanism.Methods:Aging mice model was prepared and divided into normal control group,model group,positive control group(naofukang 0.8g·kg^-1.d^-1),high,medium and low dose Indigofera stachyoides group(20g·kg^-1.d^-1、10g·kg^-1.d^-1、5g·kg^-1·d^-1).To observe the effect of Indigofera stachyoides extract on weight change and learning ability of aging mice,the contents of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)in serum of aging mice were detected by ELISA.Results:Compared with the model group,the body weight of the high-dose,medium-dose and low-dose Indigofera stachyoides group increased significantly from the second week(P<0.05).The learning ability of mice in the medium and low dose groups was significantly improved(P<0.05).Compared with the model group,the serum GSH-Px content was significantly increased in the highdose,medium-dose and low-dose groups(P<0.01).Conclusion:Indigofera stachyoides extract has obvious anti-aging effect and can significantly improve the antioxidant capacity and learning ability of aging mice.