基于UPLC-TQ MS的空心莲子草核苷类、氨基酸类成分分析

Analysis of Nucleosides and Amino Acids in Alternanthera Philoxeroides (Mart.) Griseb by UPLC-TQ MS

目的:建立空心莲子草中17种核苷类和24种氨基酸类成分的分析方法,并对空心莲子草中核苷类、氨基酸类成分进行分析。方法:采用UPLC-TQ MS法对空心莲子草中17种核苷类和24种氨基酸类成分进行含量检测,选用色谱柱Acquity UPLC BEH Amide(100mm×2.1mm,1.7μm),以A-含5mmol/L甲酸铵、乙酸铵和0.2%甲酸的水溶液,B-含1mmol/L甲酸铵、乙酸铵和0.2%甲酸的乙腈溶液为流动相梯度洗脱,多反应检测(MRM),毛细管电压3.0kV,取样锥孔电压4V,进样量为1μL,以相关标准品进行对照检测。结果:空心莲子草所有样品均含有丰富的核苷类、游离氨基酸类成分,其中核苷类成分以腺苷、尿苷、次黄嘌呤和肌苷等含量相对较高,游离氨基酸类成分含量相对较高的则依次为脯氨酸、异亮氨酸、丙氨酸和谷氨酸。结论:该研究可为空心莲子草的营养保健开发选择提供数据支撑,为其资源合理综合利用及产业化发展提供科学依据。

Objective:To establish methods for determination of 17nucleoside and 24amino acids in Alternanthera Philoxeroides(Mart.)Griseb(K).Methods:UPLC-TQ MS and HPLC method were used to analyze the content of 17nucleoside and 24amino acids in Alternanthera Philoxeroides(Mart.)Griseb(K).Column Acquity UPLC BEH Amide(100mm×2.1mm,1.7μm)and mobile phase(A-containing 5mmol/L ammonium formate,ammonium acetate and 0.2%formic acid,B-containing 1mmol/L ammonium formate,ammonium acetate and 0.2%formic acid in acetonitrile)were applied.It was eluted with multi-reaction detection(MRM).The capillary Voltage was set as 3.0kV and sampling cone voltage was 4V.The injection volume was 1μL.Results:The results showed that the analysis method established in this study is simple,accurate and reliable.All samples were rich in nucleosides and free amino acids.The contents of Adenosine,uridine,hypoxanthine and inosine were relative high in the nucleosides.For the free amino acids,high contents were found for Proline,isoleucine,alanine and glutamic acid in the samples.Conclusion:The above results provid the data support of development of Alternanthera Philoxeroides about nutrition and health and also provid a scientific basis for the rational and comprehensive utilization of resources and industrialization.