富氢水协同金复康口服液阻断硫代乙酰胺诱发大鼠胆管癌上皮癌变的实验研究

Synergistic effect of hydrogen-rich water and Jinfukang oral liquid in prevention of cholangiocarcinoma in rats induced by thioacetamide

目的观察富氢水、中成药(金复康口服液)或两者协同干预对胆管癌成瘤的影响。方法实验分为四组:对照组(n=10),富氢水组(n=10),金复康口服液组(n=5)和富氢水+金复康口服液组(n=5)。对照组予以成年SD雄性大鼠自由饮用含有硫代乙酰胺(300mg/L)水,实验组在饮用含硫代乙酰胺水的同时,分别经胃灌注适量富氢水、金复康口服液、富氢水+金复康口服液,20周处死所有大鼠,肉眼和显微镜下观察肝成瘤情况。结果9只可评价对照组大鼠在第20周时均形成肉眼以及镜下可见的肿瘤100%(9/9),而富氢水干预组7只大鼠可评价,肝肿瘤发生率为57.1%(4/7),金复康口服液干预组大鼠肝内肿瘤的成瘤率为40%(2/5),富氢水联合金复康口服液干预组大鼠肝内成瘤率为0(0/5)。结论富氢水+金复康口服液的协同作用能够成功阻断大鼠肝内胆管癌的发生。

Objective To observe the effect of hydrogen-rich water,traditional Chinese medicine(Jinfukang oral liquid,JFK)or their combined intervention on tumorigenesis of cholangiocarcinoma.Methods Adult male SD rats were divided into four groups in this study,including the control group(n=10),the hydrogen-rich water group(n=10),the JFK group(n=5)and the hydrogen-rich water+JFK group(n=5).Rats in control group were given free-drinking water containing thioacetamide(300 mg/L),while those in the experimental groups were given drinking water containing thioacetamide as well as hydrogen-rich water,JFK,or both.At 20 weeks after initiation,all rats were sacrificed,and the livers were carefully examined under the microscope.Results All ratable rats(n=9)(9/9)in the control group formed visible or microscopic tumors at 20 weeks,57.1%of rats(4/7)developed microscopic tumors in the hydrogen-rich water group,40%(2/5)developed microscopic tumors in the JFK group,while none of the rats(0/5)developed tumors in the hydrogen-rich water+JFK group.Conclusion The synergistic effect of hydrogen-enriched water and JFK can successfully block the occurrence of intrahepatic cholangiocarcinoma in rats.