瓜蒌果瓤化学成分研究

Chemical constitutes from flesh of Trichosanthes kirilowii

目的研究瓜蒌(栝楼Trichosanthes kirilowii果实)果瓤的化学成分。方法综合运用硅胶、ODS及HPLC等色谱方法对进行分离纯化,采用NMR和MS数据进行化合物的结构鉴定。结果从瓜蒌瓤95%乙醇回流提取物的醋酸乙酯萃取层得到26个化合物,分别为6个有机酸酯类:月桂酸乙酯(1)、邻苯二甲酸丁酯(2)、乙二酸二乙酯(3)、苹果酸二丁酯(4)、6,10,14,18-tetramethyl-2-ethyl-7-ene-3-hydroxyl--ninecanol-1-butyl ester(5)、drechslerol-B(6);9个有机酸及酚酸类:羟基苯甲醛(7)、水杨酸(8)、香草酸(9)、异香草酸(10)、原儿茶酸(11)、反式肉桂酸(12)、对羟基肉桂酸(13)、反式阿魏酸(14)、月桂酸(15);8个黄酮类:香叶木素(16)、芹菜素(17)、柯伊利素(18)、木犀草素(19)、4′-羟基黄芩素(20)、槲皮素(21)、香叶木素-7-O-β-D-葡萄糖苷(22)、柯伊利素-7-O-β-D-葡萄糖苷(23);2个糠醛类:5-乙酰氧甲基糠醛(24)、5-羟甲基糠醛(25)和1个环阿尔廷醇化合物cyclotucanol 3-palmitate(26)。结论除化合物10外,其他化合物均为首次从栝楼属植物中分离得到。

Objective To investigate the chemical constituents from the flesh of Trichosanthes kirilowii. Methods The chemical constituents from n-butanol fraction of 95% ethanol extract of the flesh of T. kirilowii were separated by silica gel and ODS chromatogram columns as well as preparative HPLC. On the basis of NMR and MS data analysis, their structures were elucidated. Results Twenty-six compounds were isolated from 95% ethanol extract of T. kirilowii, of which six organic esters were ethyl laurate (1), dibutyl phthalate (2), diethyl ethaneioate (3), dibutyl-2-malate (4), 6,10,14,18-tetramethyl-2-ethyl-7-ene-3-hydroxyl-ninecanol- 1-butyl ester (5), drechslerol-B (6), nine organic acids and phenolic acids were p-hydroxybenzaldehyde (7), salicylic acid (8), vanillic acid (9), isovanillic acid (10), protocatechuate (11), trans-cinnamic acid (12), p-hydroxycinnamic acid (13), trans-ferulic acid (14), and lauric acid (15), eight flavonoids were diosmetin (16), apigenin (17), chrysoeriol (18), luteolin (19), 4′-hydroxyscutellarin (20), quercetin (21), diosmetin-7-O-β-D-glucoside (22), chrysoeriol-7-O-β-D-glucoside (23), two aldehydes were 5-acetoxymethyl- 2-furaldehyde (24), 5-hydroxymethyl furfural (25) and one cycloaltinol compounds was cyclotucanol 3-palmitate (26). Conclusion All compounds except compound 10 are isolated from the Trichosanthes genus for the first time.